A comparative study on the disinfection potentials of bioactive glass S53P4 and calcium hydroxide in contra-lateral human premolars ex vivo
| Autor: | Matthias Zehnder, H. U. Luder, M. Schätzle, E. Kerosuo, Tuomas Waltimo |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Materials science Adolescent Dentistry Tryptic soy broth Enterococcus faecalis law.invention Calcium Hydroxide chemistry.chemical_compound stomatognathic system law Premolar medicine Humans Bicuspid Child General Dentistry Calcium hydroxide biology business.industry biology.organism_classification Bandages medicine.anatomical_structure chemistry Bioactive glass Sodium hypochlorite Anti-Infective Agents Local Glass Citric acid business Ex vivo |
| Zdroj: | International endodontic journal. 39(12) |
| ISSN: | 0143-2885 |
| Popis: | Aim To evaluate the effects of bioactive glass S53P4 versus calcium hydroxide when used as dressings in contra-lateral human premolars infected with Enterococcus faecalis ATCC 29212. Methodology Pairs of contra-lateral premolar teeth plus single control premolars were obtained from 23 individuals aged 10–26 years undergoing orthodontic treatment. Root canals of teeth with fully formed apices (nine contra-lateral pairs, seven controls) were instrumented using a size 60 FlexoFiles 2 mm short of canal length. Canals with open apices (six contra-lateral pairs, four controls) were circumferentially instrumented using a FlexoFile. Root canals were rinsed with 1% sodium hypochlorite and 10% citric acid. Teeth were then suspended in tryptic soy broth (TSB) and autoclaved. Positive controls and study teeth were infected with E. faecalis ATCC 29212 for 2 weeks in TSB, while negative controls were kept in sterile TSB. Subsequently, contra-lateral premolars were dressed with bioactive glass S53P4 (BAG) or calcium hydroxide suspensions for 10 days. Dentine samples were obtained from teeth with fully formed apices using ISO-size 70, 80 and 90 FlexoFiles to working length and cultured. Teeth with open apices were fixed, fractured and examined using scanning electron microscopy (SEM). Results Calcium hydroxide had a strong antibacterial effect and was significantly more effective than BAG in preventing residual bacterial growth (P |
| Databáze: | OpenAIRE |
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