Controlled hydrogel photopolymerization inside live systems by X-ray irradiation
| Autor: | Hsiang-Hisn Chen, Yeukuang Hwu, Zi-Xian Liao, Cheng-Liang Wang, Chia-Chi Chien, S.-Ja Tseng, Giorgio Margaritondo, Yi-Da Kang |
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| Přispěvatelé: | Tseng, S-Ja, Chien, Chia-Chi, Liao, Zixian, Chen, Hsiang-Hsin, Kang, YiDa, Wang, Cheng-Liang, Hwu, Yeukuang, Margaritondo, G |
| Rok vydání: | 2012 |
| Předmět: |
Polyethylenimine
Materials science Biocompatibility technology industry and agriculture cell adhesion macromolecular substances General Chemistry Condensed Matter Physics x-ray irradiation chemistry.chemical_compound Monomer Photopolymer chemistry Polymerization Chemical engineering Polymer chemistry PEG ratio Self-healing hydrogels hydrogel Ethylene glycol |
| Zdroj: | Soft Matter. 8:1420-1427 |
| ISSN: | 1744-6848 1744-683X |
| DOI: | 10.1039/c1sm06682j |
| Popis: | We successfully demonstrate the in situ formation by X-ray irradiation induced polymerization of a three-dimensional (3D) hydrogel based on poly(ethylene glycol) (PEG) diacrylate (PEG DA), with the option of heparin incorporation to enhance cell adhesion. The polymerization reaction can be completed in tens of seconds, thanks to the fast and uniform X-ray induced process. Eliminatin gpolymerization initiators and the residual unreacted monomers improves the biocompatibility and stability of the hydrogel. The photopolymerization mechanism can be locally controlled by usinga trimmed or a focused X-ray beam to allow precise in vivo administration deep in the tissue, without surgery. We also demonstrate that photopolymerized hydrogels containing polyethylenimine (PEI)/nucleic acid (DNA or small interfering RNA, siRNA) nanoparticles (NPs) can deliver such nanoparticles. The hydrogel is biocompatible with mice and is not toxic to epithelial cells. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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