Genome Editing in Agriculture
| Autor: | Rainer Fischer, Stefan Schillberg, Julia Jansing, Andreas Schiermeyer, Luisa Bortesi |
|---|---|
| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Computer science ENGINEERED NUCLEASES Review Crop species 01 natural sciences Catalysis HOMOLOGOUS RECOMBINATION Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Genome editing DNA REPLICONS oligonucleotide-directed mutagenesis Physical and Theoretical Chemistry Public acceptance Regeneration (ecology) Molecular Biology lcsh:QH301-705.5 sequence-specific nucleases Spectroscopy AGROBACTERIUM-MEDIATED TRANSFORMATION TETRAPLOID POTATO CROP IMPROVEMENT Gene Editing business.industry Organic Chemistry SELECTABLE MARKER General Medicine Computer Science Applications Plant Breeding GENETICALLY-MODIFIED PLANTS 030104 developmental biology Oligonucleotide-Directed Mutagenesis EFFICIENT TARGETED MUTAGENESIS Risk analysis (engineering) lcsh:Biology (General) lcsh:QD1-999 Agriculture base editors precision breeding programmable nucleases business DIRECTED MUTAGENESIS 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 20, Iss 12, p 2888 (2019) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20122888 |
| Popis: | The advent of precise genome-editing tools has revolutionized the way we create new plant varieties. Three groups of tools are now available, classified according to their mechanism of action: Programmable sequence-specific nucleases, base-editing enzymes, and oligonucleotides. The corresponding techniques not only lead to different outcomes, but also have implications for the public acceptance and regulatory approval of genome-edited plants. Despite the high efficiency and precision of the tools, there are still major bottlenecks in the generation of new and improved varieties, including the efficient delivery of the genome-editing reagents, the selection of desired events, and the regeneration of intact plants. In this review, we evaluate current delivery and regeneration methods, discuss their suitability for important crop species, and consider the practical aspects of applying the different genome-editing techniques in agriculture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |