Core–Shell Structured Antimicrobial Nanofiber Dressings Containing Herbal Extract and Antibiotics Combination for the Prevention of Biofilms and Promotion of Cutaneous Wound Healing
| Autor: | Hariharan Ezhilarasu, Seow Theng Ong, Chak Ming Leung, Praseetha Prasannan, Mohammed Kamruddin, Venkatesh Mayandi, Kantha Deivi Arunachalam, Nandhini Sunderasan, Sathish Kumar Karuppannan, Seeram Ramakrishna, Veluchamy A Barathi, Rajamani Lakshminarayanan, Ilango Kaliappan, Raghavendra Ramalingam, Chetna Dhand, Navin Kumar Verma |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
food.ingredient Swine medicine.drug_class Herbal Medicine Exogenous bacteria Antibiotics Nanofibers 02 engineering and technology Gelatin Bacterial Adhesion 03 medical and health sciences food Anti-Infective Agents medicine Animals Humans General Materials Science Cell Proliferation Skin 030304 developmental biology Wound Healing 0303 health sciences biology Traditional medicine Minocycline Hydrochloride Minocycline 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification Bandages Biofilms Nanofiber Gymnema sylvestre 0210 nano-technology medicine.drug |
| Zdroj: | ACS Applied Materials & Interfaces. 13:24356-24369 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Burn wounds are susceptible to microbial invasion from both resident and exogenous bacteria, which becomes a critical public health issue and causes substantial economic burden. There is a perceived demand to produce new antimicrobial wound dressings that hinder bacterial colonization while accelerating the healing process and hence would provide an improved standard of care for patients. Since ancient times, herbal extracts from medicinally important plants have extensively been used for treating burn injuries. This work reports the utility of electrospun nanofibers containing plant extracts and antibiotics combination as a multifunctional scaffold for treating second-degree burns. First, we determined the various components of plant extracts from Gymnema sylvestre by two different processing methods and their synergism with minocycline antibiotics. Then, we prepared core-shell nanofibrous dressings with poly-e-caprolactone/gelatin laden with minocycline hydrochloride as a shell and gelatin infused with G. sylvestre extracts (ultrasound-assisted extracts and cold macerated extracts) as the core using coaxial electrospinning. The electrospun nanofibers displayed a smooth, continuous, and bead-free morphology with adequate wettability. The presence of extract components in the core-shell nanofibers resulted in enhanced mechanical properties when compared to pristine mats. The core-shell structures resulted in sustained release of the bioactive components when compared to nanofiber blends. Core-shell nanofiber mats containing plant extracts and antibiotic combinations displayed potent antimicrobial and antibiofilm properties while promoting the spread and proliferation of skin cells when compared to pristine mats. In a porcine model of cutaneous second-degree burns, we showed that wounds treated with the antimicrobial dressing improved re-epithelialization and collagen organization in comparison to untreated wounds. |
| Databáze: | OpenAIRE |
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