Robust and Highly-Efficient Differentiation of Functional Monocytic Cells from Human Pluripotent Stem Cells under Serum- and Feeder Cell-Free Conditions
| Autor: | Shota Tomida, Takayuki Tanaka, Takahiro Yasumi, Hiroaki Goto, Tatsutoshi Nakahata, Seiko Nishimoto, Toshio Heike, Yuuki Murata, Isao Asaka, Koichi Oshima, Akira Niwa, Jun Ito, Fumiko Honda-Ozaki, Masakatsu Yanagimachi, Megumu K. Saito |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cellular differentiation
Immune Cells Induced Pluripotent Stem Cells Immunology Cell Culture Techniques lcsh:Medicine Antigen-Presenting Cells Biology Regenerative medicine Cell Fate Determination Culture Media Serum-Free Monocytes Immune system Humans lcsh:Science Induced pluripotent stem cell Cells Cultured Embryonic Stem Cells Multidisciplinary Immunomagnetic Separation Macrophages Stem Cells lcsh:R Cell Differentiation Dendritic Cells Embryonic stem cell Antigens Differentiation Cell biology Endothelial stem cell Phenotype Cell culture Cytokines lcsh:Q Stem cell Stem Cell Lines Research Article Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 4, p e59243 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Monocytic lineage cells (monocytes, macrophages and dendritic cells) play important roles in immune responses and are involved in various pathological conditions. The development of monocytic cells from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is of particular interest because it provides an unlimited cell source for clinical application and basic research on disease pathology. Although the methods for monocytic cell differentiation from ESCs/iPSCs using embryonic body or feeder co-culture systems have already been established, these methods depend on the use of xenogeneic materials and, therefore, have a relatively poor-reproducibility. Here, we established a robust and highly-efficient method to differentiate functional monocytic cells from ESCs/iPSCs under serum- and feeder cell-free conditions. This method produced 1.3 × 10(6) ± 0.3 × 10(6) floating monocytes from approximately 30 clusters of ESCs/iPSCs 5-6 times per course of differentiation. Such monocytes could be differentiated into functional macrophages and dendritic cells. This method should be useful for regenerative medicine, disease-specific iPSC studies and drug discovery. |
| Databáze: | OpenAIRE |
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