Perinatal exposure to Bisphenol A disturbs the early differentiation of male germ cells
| Autor: | María Belén Harreguy, Maria Laura Zenclussen, Mariela Bollati-Fogolín, Romina Pagotto, Martina Crispo, Horacio A. Rodríguez, Clarisa Guillermina Santamaria, Julián Elías Abud, Laura Kass, Mónica Muñoz-de-Toro |
|---|---|
| Přispěvatelé: | Institut Pasteur de Montevideo, Réseau International des Instituts Pasteur (RIIP), Instituto de Agrobiotecnología del Litoral [Santa Fe] (IAL), Universidad Nacional del Litoral [Santa Fe] (UNL)-Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Transgenic and Experimental Animal / Animales Transgénicos y de Experimentación [Montevideo], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Genetically modified mouse endocrine system [SDV]Life Sciences [q-bio] Mice Transgenic Endocrine Disruptors 010501 environmental sciences Biology Toxicology 01 natural sciences Green fluorescent protein Andrology 03 medical and health sciences Phenols Pregnancy Testis medicine Animals Benzhydryl Compounds Maternal-Fetal Exchange Cell Proliferation 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Sperm Count Perinatal Exposure urogenital system SOXB1 Transcription Factors Cell Differentiation Epididymis Embryonic stem cell Sperm Androgen receptor Germ Cells medicine.anatomical_structure Receptors Androgen Prenatal Exposure Delayed Effects Sperm Motility Female Octamer Transcription Factor-3 Germ cell |
| Zdroj: | Reproductive Toxicology Reproductive Toxicology, Elsevier, 2020, 98, pp.117-124. ⟨10.1016/j.reprotox.2020.09.004⟩ |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2020.09.004⟩ |
| Popis: | Understanding the effects of Bisphenol A (BPA) on early germ cell differentiation and their consequences in adult life is an area of growing interest in the field of endocrine disruption. Herein, we investigate whether perinatal exposure to BPA affects the differentiation of male germ cells in early life using a transgenic mouse expressing the GFP reporter protein under the Oct4 promoter. In this model, the expression of GFP reflects the expression of the Oct4 gene. This pluripotency gene is required to maintain the spermatogonial stem cells in an undifferentiated stage. Thus, GFP expression was used as a parameter to evaluate the effect of BPA on early germ cell development. Female pregnant transgenic mice were exposed to BPA by oral gavage, from embryonic day 5.5 to postnatal day 7 (PND7). The effects of BPA on male germ cell differentiation were evaluated at PND7, while sperm quality, testicular morphology, and protein expression of androgen receptor and proliferating cell nuclear antigen were studied at PND130. We found that perinatal/lactational exposure to BPA up-regulates the expression of Oct4-driven GFP in testicular cells at PND7. This finding suggests a higher proportion of undifferentiated spermatogonia in BPA-treated animals compared with non-exposed mice. Moreover, in adulthood, the number of spermatozoa per epididymis was reduced in those animals perinatally exposed to BPA. This work shows that developmental exposure to BPA disturbed the normal differentiation of male germ cells early in life, mainly by altering the expression of Oct4 and exerted long-lasting sequelae at the adult stage, affecting sperm count and testis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect