Effects of oligosaccharides and polysaccharides on the generation of reactive oxygen species in different biological systems
| Autor: | Romeo Radman, Elliot James Bland, Tajalli Keshavarz, Nuntaka Sangworachat, Christopher Bucke |
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| Rok vydání: | 2006 |
| Předmět: |
Neutrophils
Biomedical Engineering Oligosaccharides Bioengineering Penicillium chrysogenum Polysaccharide Applied Microbiology and Biotechnology Microbiology Polysaccharides Drug Discovery Humans Overproduction Cells Cultured Mannan chemistry.chemical_classification Reactive oxygen species biology Streptomycetaceae Process Chemistry and Technology food and beverages Streptomyces rimosus General Medicine Oligosaccharide biology.organism_classification Streptomyces chemistry Biochemistry Molecular Medicine Reactive Oxygen Species Biotechnology |
| Zdroj: | Biotechnology and Applied Biochemistry. 44:129 |
| ISSN: | 1470-8744 0885-4513 |
| DOI: | 10.1042/ba20050217 |
| Popis: | Use of carbohydrates as elicitors is a novel technique for enhancement of the production of industrially important microbial products. The relation between the levels of ROS (reactive oxygen species) and overproduction of antibiotics in microbial cultures has already been established. In the present study, we aimed to exploit the ROS response to develop a fast technique to assess the potential of oligosaccharides [oligoguluronate, oligomannuronate and MO (mannan oligosaccharides)] and polysaccharides [alginate and LBG (locust-bean gum)] as elicitors for overproduction of secondary metabolites in Streptomyces rimosus and Penicillium chrysogenum. We have also investigated changes in the production of ROS in neutrophils as a result of the action of the same elicitors. LBG-derived oligosaccharides (MO) were most potent inhibitors of ROS in all systems investigated. This correlates with overproduction of secondary metabolites in microbes and enhancement of a number of mammalian systems. We believe that the effects of oligosaccharides and polysaccharides on ROS production by mammalian and microbial cells can be correlated predicatively with overproduction. The underlying methodology offers a fast screening of elicitors that can be applied across the different systems. |
| Databáze: | OpenAIRE |
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