Neutrophil migration into the bovine uterine lumen following intrauterine inoculation with killed Haemophilus somnus
| Autor: | P. L. Senger, P R Widders, B. M. Butt |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Embryology
Neutrophils animal diseases medicine.medical_treatment Haemophilus Uterus Biology Microbiology Endocrinology Estrus Cell Movement medicine Animals Saline Estrous cycle Inoculation Pasteurellaceae Obstetrics and Gynecology Cell Biology Haemophilus somnus biology.organism_classification Kinetics medicine.anatomical_structure Reproductive Medicine Effusion In utero Bacterial Vaccines Cattle Female |
| Zdroj: | Reproduction. 93:341-345 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0930341 |
| Popis: | Polymorphonuclear neutrophils (PMN) in bovine uterine flushings following intrauterine deposition of killed bacteria were measured and the effect of immune status on the influx of PMN into the uterine lumen during oestrus was determined. Holstein heifers were immunized with a 270-kDa outer-membrane protein (omp-270) from Haemophilus somnus. During oestrus, immunized heifers (n = 21) received an intrauterine inoculum of either a heat-killed suspension of a homologous strain of H. somnus containing omp-270 (n = 7), a heterologous strain of H. somnus lacking omp-270 (n = 7), or phosphate-buffered saline (n = 7). Five additional heifers were inseminated with extended bovine semen. Uterine contents were collected in saline lavage immediately before inoculation (t0) and at 6, 24, 48, 72, 96, and 120 h after inoculation. The semen-inoculated heifers were lavaged only at t120. All groups experienced PMN infiltration which peaked 6 h after inoculation and tended to decline thereafter. Differences were not observed between treatment groups, indicating that neither bacterial inoculation nor immune status was as important in eliciting PMN effusion as the flushing procedure itself. |
| Databáze: | OpenAIRE |
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