Disposable screen-printed electrode coupled with recombinant Drosophila melanogaster acetylcholinesterase and multiwalled carbon nanotubes for rapid detection of pesticides
| Autor: | Dabing Zhang, Aibo Wu, Shiping(宋世平）) Song, Chunhai（樊春海） Fan, Zhuzhao Tang, Haode Chen |
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| Rok vydání: | 2011 |
| Předmět: |
Immobilized enzyme
Food Contamination Biosensing Techniques Analytical Chemistry chemistry.chemical_compound Carbofuran Dichlorvos Environmental Chemistry Animals Drosophila Proteins Pesticides Disposable Equipment Electrodes Pharmacology Prussian blue Chromatography Pesticide residue Nanotubes Carbon Organophosphate Enzymes Immobilized Recombinant Proteins Drosophila melanogaster chemistry Acetylcholinesterase Printing Cattle Glutaraldehyde Agronomy and Crop Science Biosensor Water Pollutants Chemical Food Science Ferrocyanides |
| Zdroj: | Journal of AOAC International. 94(1) |
| ISSN: | 1060-3271 |
| Popis: | Recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE), multiwalled carbon nanotubes (MWCNTs), and Prussian blue have been combined for development of a three-electrode biosensor with more rapid responses and higher stability than in our previous study. A new disposable screen-printed electrode (SPE) was developed for rapid detection of organophosphate and carbamate pesticides. After optimization, 10 μg MWCNT and 5 μL enzyme immobilization solution consisting of 0.2% glutaraldehyde, 0.1% Nafion®, 0.2% bovine serum albumin, 0.1 g/L MWCNT, and 1.5 mU R-DmAChE were fixed on each of the R-DmAChE/MWCNT SPEs. The LOD of this biosensor was 0.5 μg/L for pesticide standards of dichlorvos (DDV) and carbofuran. The performance of this biosensor was tested for vegetable and water samples at various spiked levels, and good stability and sensitivity were found. The obtained recoveries were from 82.6 to 110.5% for DDV at levels of 0.5–5 μg/L and 73.4 to 118.4% for carbofuran at 1–10 μg/L in lake and sea water samples, demonstrating that the proposed approach is an alternative means for rapid detection of pesticide residues and contaminants in food safety and environmental monitoring. |
| Databáze: | OpenAIRE |
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