Long non-coding RNA GAS5 promotes PC12 cells differentiation into Tuj1-positive neuron-like cells and induces cell cycle arrest
| Autor: | Sheng-Tong Zhang, Lei Zhang, Jianbing Qin, Yan Sun, Wei-Ye Zhang, Haoming Li, Hui He, Xiang Cheng, Heyan Zhao, Guohua Jin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
proliferation growth arrest-specific 5 lcsh:RC346-429 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience medicine nerve regeneration lcsh:Neurology. Diseases of the nervous system PC12 cell biology Cell growth Chemistry neuron cell cycle choline acetyltransferase acetylcholine Alzheimer′s disease neural regeneration Transfection Cell cycle Alzheimer's disease Choline acetyltransferase Doublecortin Cell biology 030104 developmental biology medicine.anatomical_structure nervous system biology.protein Cholinergic Neuron 030217 neurology & neurosurgery Acetylcholine medicine.drug Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 14, Iss 12, Pp 2118-2125 (2019) |
| ISSN: | 1876-7958 1673-5374 |
| Popis: | Growth arrest-specific 5 (GAS5) is an anti-oncogene that has been extensively studied in tumors. However, research on GAS5 in the context of nervous system disease is rare at present. This study aimed to investigate the role of the long non-coding RNA GAS5 in rat pheochromocytoma cells (PC12 cells). GAS5-overexpressing lentivirus was transfected into PC12 cells, and expression levels of GAS5 and C-myc were detected by real-time PCR. Ratios of cells in S phase were detected by 5-ethynyl-2'-deoxyuridine. Immunohistochemical staining was used to detect the immunoreactivity of neuron microtubule markers Tuj1, doublecortin, and microtubule-associated protein 2. Apoptosis was detected by flow cytometry, while expression of acetylcholine in cells was detected by western blot assay. We found that GAS5 can promote PC12 cells to differentiate into Tuj1-positive neuron-like cells with longer processes. In addition, cell proliferation and cell cycle were significantly suppressed by GAS5, whereas it had no effect on apoptosis of PC12 cells. Our results indicate that GAS5 could increase the expression of choline acetyltransferase and acetylcholine release. Thus, we speculate that GAS5 is beneficial to the recovery of neurons and the cholinergic nervous system. |
| Databáze: | OpenAIRE |
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