Prediction of colorectal cancer diagnosis based on circulating plasma proteins

Autor: Ching Yun Chang, Ruedi Aebersold, Timothy Clough, Peter J. Schüffler, Josef Srovnal, Sha Tao, Mariette Matondo, Meena Choi, Yansheng Liu, Kamil Vysloužil, Silvia Surinova, Olga Vitek, Joachim M. Buhmann, Marta Khoylou, Hendrik Weisser, Hermann Brenner, Marian Hajduch, Ruth Hüttenhain
Přispěvatelé: Institute of Molecular Systems Biology [Zurich], Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Purdue University [West Lafayette], Division of Clinical Epidemiology and Aging Research, German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), Institute for Machine Learning [Zurich], Department of Computer Science [ETH Zürich] (D-INFK), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich)- Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), University Hospital Olomouc [Czech Republic], Faculty of Medicine and Dentistry [Olomouc, Czech Republic], Palacky University Olomouc, German Cancer Consortium [Heidelberg] (DKTK), Northeastern University [Boston], Universität Zürich [Zürich] = University of Zurich (UZH), This work was supported by funding from the Swiss National Science Foundation (grant # 3100A0‐107679, to R.A.)., We would like to thank Ralph Schiess for helpful discussions and enthusiasm throughout the project
Jazyk: angličtina
Rok vydání: 2015
Předmět:
discovery-driven and targeted proteomics
Colorectal cancer
[SDV]Life Sciences [q-bio]
Cell
discovery‐driven and targeted proteomics
colorectal cancer
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Bioinformatics
Systemic circulation
Mass Spectrometry
Plasma
Blood plasma
Biomarkers
Tumor
medicine
Humans
MESH: Mass Spectrometry/methods
Research Articles
chemistry.chemical_classification
MESH: Biomarkers
Tumor/blood
MESH: Clinical Laboratory Techniques/methods
MESH: Humans
Clinical Laboratory Techniques
business.industry
MESH: Plasma/chemistry
MESH: Colorectal Neoplasms/diagnosis
medicine.disease
Primary tumor
Blood proteins
3. Good health
Biomarker (cell)
diagnostic protein biomarker
medicine.anatomical_structure
chemistry
Cancer research
Molecular Medicine
Colorectal Neoplasms
Glycoprotein
business
Zdroj: EMBO Molecular Medicine
EMBO Molecular Medicine, Wiley Open Access, 2015, 7 (9), pp.1166-1178. ⟨10.15252/emmm.201404873⟩
ISSN: 1757-4676
1757-4684
Popis: International audience; Non-invasive detection of colorectal cancer with blood-based markers is a critical clinical need. Here we describe a phased mass spectrometry-based approach for the discovery, screening, and validation of circulating protein biomarkers with diagnostic value. Initially, we profiled human primary tumor tissue epithelia and characterized about 300 secreted and cell surface candidate glycoproteins. These candidates were then screened in patient systemic circulation to identify detectable candidates in blood plasma. An 88-plex targeting method was established to systematically monitor these proteins in two large and independent cohorts of plasma samples, which generated quantitative clinical datasets at an unprecedented scale. The data were deployed to develop and evaluate a five-protein biomarker signature for colorectal cancer detection.
Databáze: OpenAIRE
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