mTHPC-mediated Photodynamic Treatment Up-regulates the Cytokines VEGF and IL-1alpha
| Autor: | Vaclovas Jurgelevicius, Neringa Daugelaviciene, Daiva Dabkeviciene, Ausra Sasnauskiene, Vida Kirveliene, Benediktas Juodka, Vaidotas Stankevicius, Eva Leman, Raimonda Kvietkauskaite |
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| Rok vydání: | 2012 |
| Předmět: |
Vascular Endothelial Growth Factor A
Small interfering RNA Light medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Photodynamic therapy Biology Polymerase Chain Reaction Biochemistry chemistry.chemical_compound Cell Line Tumor Interleukin-1alpha medicine Humans Gene silencing Cytotoxic T cell Gene Silencing RNA Small Interfering Physical and Theoretical Chemistry Photosensitizing Agents General Medicine Molecular biology Up-Regulation Gene Expression Regulation Neoplastic Vascular endothelial growth factor Real-time polymerase chain reaction Mesoporphyrins Photochemotherapy chemistry Epidermoid carcinoma Cell culture Carcinoma Squamous Cell Cancer research Signal Transduction |
| Zdroj: | Photochemistry and Photobiology. 88:432-439 |
| ISSN: | 0031-8655 |
| DOI: | 10.1111/j.1751-1097.2011.01062.x |
| Popis: | Photodynamic therapy (PDT) of cancer induces oxidative stress, which intervenes in the expression of cytokines by tumor cells. The cytokines might have either a positive or a negative impact on tumor eradication. Here, we studied the expression of cytokines vascular endothelial growth factor (VEGF) and interleukin-1alpha (IL-1alpha) in the human epidermoid carcinoma A-431 cells following m-tetra(3-hydroxyphenyl)-chlorin (mTHPC)-mediated PDT in vitro and assessed the IL-1alpha effect on VEGF expression. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay revealed the enhanced production of VEGF and IL-1alpha both on mRNA and protein levels by mTHPC-loaded cells after light exposure. The silencing of IL1A by small interfering RNA resulted in decreased production of IL-1alpha and a reduced amount of VEGF. Furthermore, exogenous recombinant IL-1alpha stimulated the VEGF expression after PDT. Thus, in addition to the cytotoxic action on the A-431 cells, mTHPC-mediated PDT stimulated the production of VEGF and IL-1alpha, and IL-1alpha contributed to the VEGF overexpression. These data establish IL-1alpha as a possible target of combined cancer treatment. |
| Databáze: | OpenAIRE |
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