Intravital microscopic optical coherence tomography imaging to assess mucus-mobilizing interventions for muco-obstructive lung disease in mice
| Autor: | Gereon Hüttmann, Hinnerk Schulz-Hildebrandt, Peter König, Marcus A. Mall, Mario Pieper |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Intravital Microscopy muco-obstructive disease Physiology medicine.medical_treatment Mice Transgenic Cystic fibrosis Mice 03 medical and health sciences 0302 clinical medicine In vivo Physiology (medical) medicine Animals Humans Lung Diseases Obstructive Epithelial Sodium Channels Saline mucus-mobilizing therapy COPD optical coherence tomography Lung business.industry Cell Biology respiratory system medicine.disease Mucus Obstructive lung disease Hypertonic saline Disease Models Animal 030104 developmental biology medicine.anatomical_structure 030228 respiratory system Mucociliary Clearance Innovative Methodology intravital imaging business Tomography Optical Coherence |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology |
| ISSN: | 1522-1504 1040-0605 |
| Popis: | Airway mucus obstruction is a hallmark of chronic lung diseases such as cystic fibrosis, asthma, and COPD, and the development of more effective mucus-mobilizing therapies remains an important unmet need for patients with these muco-obstructive lung diseases. However, methods for sensitive visualization and quantitative assessment of immediate effects of therapeutic interventions on mucus clearance in vivo are lacking. In this study, we determined whether newly developed high-speed microscopic optical coherence tomography (mOCT) is sensitive to detect and compare in vivo effects of inhaled isotonic saline, hypertonic saline, and bicarbonate on mucus mobilization and clearance in Scnn1b-transgenic mice with muco-obstructive lung disease. In vivo mOCT imaging showed that inhaled isotonic saline-induced rapid mobilization of mucus that was mainly transported as chunks from the lower airways of Scnn1b-transgenic mice. Hypertonic saline mobilized a significantly greater amount of mucus that showed a more uniform distribution compared with isotonic saline. The addition of bicarbonate-to-isotonic saline had no effect on mucus mobilization, but also led to a more uniform mucus layer compared with treatment with isotonic saline alone. mOCT can detect differences in response to mucus-mobilizing interventions in vivo, and may thus support the development of more effective therapies for patients with muco-obstructive lung diseases. |
| Databáze: | OpenAIRE |
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