Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations
| Autor: | Abdennaceur Hassen, Sana Kefacha, Abdelwaheb Chatti, Moncef Feki, M.M. Sethom, Mouadh Mihoub, Salma Kloula Ben Ghorbal, Ahmed Landoulsi, Lobna Maalej |
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| Rok vydání: | 2013 |
| Předmět: |
Chromatography
Gas Ultraviolet Rays Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Stress Physiological medicine Cardiolipin Unsaturated fatty acid Dichloromethane Phosphatidylethanolamine Phosphatidylglycerol Chromatography Pseudomonas aeruginosa Cell Membrane Fatty Acids Homeoviscous adaptation General Medicine Membrane chemistry Biochemistry lipids (amino acids peptides and proteins) Chromatography Thin Layer |
| Zdroj: | Current Microbiology. 67:112-117 |
| ISSN: | 1432-0991 0343-8651 |
| Popis: | The changes in lipid composition enable the micro-organisms to maintain membrane functions in the face of environmental fluctuations. The relationship between membrane fatty acid composition and UV-C stress was determined for mid-exponential phase and stationary phase Pseudomonas aeruginosa. The total lipids were obtained by dichloromethane/methanol (3:1) and were quantified by GC. The TLC analysis of phospholipids showed the presence of three major fractions phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. Significant modifications, as manifested by an increase of UFA, were obtained. Interestingly, this microorganism showed a remarkable capacity for recovery from the stressful effects of UV-C. |
| Databáze: | OpenAIRE |
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