High resolution and rapid separation of bacteria from blood using elasto‐inertial microfluidics
| Autor: | Aman Russom, Sharath Narayana Iyengar, Gustaf Mårtensson, Tharagan Kumar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Resolution (mass spectrometry) Microfluidics Separation (aeronautics) Clinical Biochemistry 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Sepsis Analytisk kemi Humans Sample preparation Spiral Blood Cells Chromatography Bacteria biology 010401 analytical chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology biology.organism_classification Non-Newtonian fluid 0104 chemical sciences Dilution 0210 nano-technology Inertial microfluidics |
| Popis: | Improved sample preparation has the potential to address unmet needs for fast turnaroundsepsis tests. In this work, we report elasto-inertial based rapid bacteria separation from diluted blood at high separation efficiency. In viscoelastic flows, we demonstrate novel findings where blood cells prepositioned at the outer wall entering a spiral device remain fullyfocused throughout the channel length while smaller bacteria migrate to the opposite wall.Initially, using microparticles, we show that particles above a certain size cut-off remainfully focused at the outer wall while smaller particles differentially migrate toward the inner wall. We demonstrate particle separation at 1 μm resolution at a total throughput of1 mL/min. For blood-based experiments, a minimum of 1:2 dilution was necessary to fullyfocus blood cells at the outer wall. Finally, Escherichia coli spiked in diluted blood were continuously separated at a total flow rate of 1 mL/min, with efficiencies between 82 and 90%depending on the blood dilution. Using a single spiral, it takes 40 min to process 1 mLof blood at a separation efficiency of 82%. The label-free, passive, and rapid bacteria isolation method has a great potential for speeding up downstream phenotypic and genotypicanalysis. QC 20220426 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|