Synovial TRPV1 is upregulated by 17-β-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats

Autor: Yu-Wei Wu, Xiao-Xing Kou, Xu-chen Ma, Ye-Hua Gan, Ting Hao
Rok vydání: 2015
Předmět:
Freund's Adjuvant
Arthritis
Rats
Sprague-Dawley
Random Allocation
chemistry.chemical_compound
Nerve Growth Factor
Medicine
Estradiol
Temporomandibular Joint
General Medicine
Temporomandibular Joint Disorders
Up-Regulation
Allodynia
medicine.anatomical_structure
Hyperalgesia
Anesthesia
Female
lipids (amino acids
peptides
and proteins)
medicine.symptom
Capsazepine
psychological phenomena and processes
Transcriptional Activation
Agonist
medicine.medical_specialty
Orofacial pain
medicine.drug_class
Ovariectomy
Blotting
Western
TRPV1
TRPV Cation Channels
Real-Time Polymerase Chain Reaction
stomatognathic system
Internal medicine
Animals
General Dentistry
Inflammation
business.industry
Cell Biology
medicine.disease
Rats
stomatognathic diseases
Nerve growth factor
Endocrinology
nervous system
Otorhinolaryngology
chemistry
Cyclooxygenase 2
Capsaicin
Synovial membrane
business
Zdroj: Archives of Oral Biology. 60:1310-1318
ISSN: 0003-9969
DOI: 10.1016/j.archoralbio.2015.05.011
Popis: Women with reproductive capability are more likely to suffer from temporomandibular disorders (TMD), with orofacial pain as the most common complaint. In the past, we focused on the role of estradiol in TMD pain through the nervous system. In this study, we explored estradiol's influence on synoviocyte gene expressions involved in the allodynia of the inflamed TMJ. The influence of 17-β-estradiol on NGF and TRPV1 expression in TMJ synovium was determined in vivo and in vitro and analyzed by Western blot and real-time PCR. Complete Freund's adjuvant (CFA) injection into the TMJ was used to induce TMJ arthritis. Capsazepine served as a TRPV1 antagonist. Head withdrawal threshold was examined using a von Frey Anesthesiometer. We observed that estradiol upregulated the expressions of TRPV1 and NGF in a dose-dependent manner. In the primary cultured synoviocytes, TRPV1 was upregulated by lipopolysaccharide (LPS), estradiol, and NGF, while NGF antibodies fully blocked LPS and estradiol-induced upregulation of TRPV1. Activation of TRPV1 in the primary synoviocytes with capsaicin, a TRPV1 agonist, dose-dependently enhanced COX-2 transcription. Moreover, intra-TMJ injection of TRPV1 antagonist, capsazepine, significantly attenuated allodynia of the inflamed TMJ induced by intra-TMJ injection of CFA in female rats. This article presents a possible local mechanism for estradiol that may be involved in TMJ inflammation or pain in the synovial membrane through the pain-related gene TRPV1. This finding could potentially help clinicians understand the sexual dimorphism of TMD pain.
Databáze: OpenAIRE
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