Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli
| Autor: | N H Giles, A R Hawkins, W R Reinert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1982 |
| Předmět: |
N crassa
medicine.disease_cause Biochemistry Neurospora crassa medicine Escherichia coli Amino Acid Sequence Molecular Biology Gene Hydro-Lyases chemistry.chemical_classification biology Catabolism fungi Crassa Cell Biology biology.organism_classification Kinetics Neurospora Enzyme chemistry Homogeneous Research Article |
| Popis: | 1. Neurospora crassa catabolic dehydroquinase has been purified from N. crassa and Escherichia coli. 2. Protein-sequence and gel-electrophoretic data show that apparently pure, homogeneous native dehydroquinase is a mixture of intact and proteinase-cleaved enzyme monomers. 3. Protein-sequence data and steady-state kinetics show that the catabolic dehydroquinase gene of N. crassa is expressed with fidelity in E. coli. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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