Fractionation of milk proteins on pilot scale with particular focus on β-casein
| Autor: | Aline Holder, Thomas Schubert, Remko M. Boom, Katharina J.F. Thienel, Zeynep Atamer, Jörg Hinrichs |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Chromatography
Precipitation (chemistry) Chemistry Extraction (chemistry) 0402 animal and dairy science Pilot scale Fraction (chemistry) 04 agricultural and veterinary sciences Fractionation 040401 food science 040201 dairy & animal science Applied Microbiology and Biotechnology 0404 agricultural biotechnology β casein Casein Yield (chemistry) Life Science Food Process Engineering Food Science VLAG |
| Zdroj: | International Dairy Journal, 79, 73-77 International Dairy Journal 79 (2018) |
| ISSN: | 0958-6946 |
| Popis: | The aim of this study was to increase the yield and purity of casein fractions at pilot scale and determine the main process parameters influencing the isolation of β-casein, such as cold extraction time, separation speed. The fractions were obtained from micellar casein by means of selective precipitation following separation by a nozzle centrifuge. The purities, which were calculated based on the total casein content of the β-casein fraction, and the yields achieved for β-casein ranged from 68.7 to 89.6% and from 10 to 32%, respectively. For the other two fractions, αS- and κ-casein, the obtained purities were 61% and 43%, respectively. Using the proposed isolation method, high purities for the β-casein fraction were achievable; however, the other two fractions, αS- and κ-casein, need further improvement. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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