Donut-shaped 'miniparticles' in nuclei of human and rat cells
| Autor: | William H. Spohn, Fred R. Harmon, Rose K. Busch, Chirala S. Subrahmanyam, Naochika Domae, Harris Busch |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Cell Nucleus
Two-dimensional gel electrophoresis Lymphoma Particle number Density gradient Chemistry Placenta Analytical chemistry General Medicine Electron Cell Fractionation Weight range General Biochemistry Genetics and Molecular Biology Rats Sedimentation coefficient Microscopy Electron Cytosol Liver Pregnancy Centrifugation Density Gradient Animals Humans Female General Pharmacology Toxicology and Pharmaceutics Quantitative analysis (chemistry) |
| Zdroj: | Life Sciences. 30:469-477 |
| ISSN: | 0024-3205 |
| Popis: | Donut-shaped “miniparticles” were extracted from nuclei of various types of human and rat cells. Electron-microscopic investigations showed these particles were predominantly in sucrose density gradient fractions that had an approximate sedimentation coefficient of 21S. These particles were 113±8 A o in diameter and had an electron dense center of 29±6 A o . They appeared to be composed of 8 subunits. Quantitative analysis of the number of these particles by electron-micrographic field counting showed nuclei of tumor samples had a larger amount of the particles than the cytosol. However, normal cell cytosol had a larger number of particles than the nuclei. A group of proteins in the 25, 000–33, 000 molecular weight range was shown to be the main protein component by two dimensional gel electrophoresis. |
| Databáze: | OpenAIRE |
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