Multimodal fluorescence lifetime imaging and optical coherence tomography for longitudinal monitoring of tissue-engineered cartilage maturation in a preclinical implantation model
| Autor: | Zhou, Xiangnan, Haudenschild, Anne K, Li, Cai, Marcu, Laura |
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| Rok vydání: | 2023 |
| Předmět: |
Aging
optical coherence tomography Tissue Engineering engineered tissues Nude Biomedical Engineering Bioengineering Optics Optical Physics Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Biomaterials Mice Cartilage Optical Coherence Opthalmology and Optometry fluorescence lifetime Animals Biomedical Imaging Collagen Tomography tissue optical spectroscopy and imaging Biotechnology |
| Zdroj: | Journal of biomedical optics, vol 28, iss 2 |
| ISSN: | 1083-3668 |
| Popis: | SignificanceCartilage tissue engineering is a promising strategy for effective curative therapies for treatment of osteoarthritis. However, tissue engineers depend predominantly on time-consuming, expensive, and destructive techniques as quality control to monitor the maturation of engineered cartilage. This practice can be impractical for large-scale biomanufacturing and prevents spatial and temporal monitoring of tissue growth, which is critical for the fabrication of clinically relevant-sized cartilage constructs. Nondestructive multimodal imaging techniques combining fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) hold great potential to address this challenge.AimThe feasibility of using multimodal FLIm-OCT for nondestructive, spatial, and temporal monitoring of self-assembled cartilage tissue maturation in a preclinical mouse model is investigated.ApproachSelf-assembled cartilage constructs were developed for 4 weeks in vitro followed by 4 weeks of in vivo maturation in nude mice. Sterile and nondestructive in situ multispectral FLIm and OCT imaging were carried out at multiple time points ( t=2 , 4, and 8 weeks) during tissue development. FLIm and 3D volumetric OCT images were reconstructed and used for the analysis of tissue biochemical homogeneity, morphology, and structural integrity. A biochemical homogeneity index was computed to characterize nonhomogeneous tissue growth at different time points. OCT images were validated against histology.ResultsFLIm detects heterogenous extracellular matrix (ECM) growth of tissue-engineered cartilage. The outer edge of the tissue construct exhibited longer fluorescence lifetime in 375 to 410 and 450 to 485nm spectral channels, indicating increase in collagen content. Significant ( p |
| Databáze: | OpenAIRE |
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