Characterization of a yeast D-amino acid oxidase microbiosensor for D-serine detection in the central nervous system
| Autor: | Pierre Pernot, Jean-Pierre Mothet, Oleg Schuvailo, Alexey Soldatkin, Loredano Pollegioni, Mirella Pilone, Marie-Thérèse Adeline, Raymond Cespuglio, Stéphane Marinesco |
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| Přispěvatelé: | Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS), CNRS, Institut de Neurobiologie Alfred Fessard-FRC2118, Laboratoire de Neurobiologie Cellulaire et Moléculaire-UPR9040, Physiopathologie du système nerveux central - Institut François Magendie, Université Bordeaux Segalen - Bordeaux 2-IFR8-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine (NASU), Radicaux Libres, Substrats Énergétiques et Physiopathologie Cérébrale, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Department of Biotechnology and Molecular Sciences, Universitá degli Studi dell’Insubria, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Physiologie integrée du système d'éveil, Université de Lyon-Université de Lyon-IFR19-Institut National de la Santé et de la Recherche Médicale (INSERM), Fondation pour la Recherche Médicale (bourse de retour 2004), Marie Curie Program (MIRG-CT-2005-017196 – SERELAS), Agence Nationale pour la Recherche (ANR-06-EMPB-029, SERICAP), NATO Collaborative Linkage Grant (CBP.NUKR.CLG 982788), EGIDE ECO-NET program (#126758G), Marinesco, Stephane, Universitá degli Studi dell’Insubria = University of Insubria [Varese] (Uninsubria), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Central Nervous System
D-Amino-Acid Oxidase [CHIM.ANAL] Chemical Sciences/Analytical chemistry [SDV.BIO]Life Sciences [q-bio]/Biotechnology D-amino acid oxidase Biosensing Techniques Analytical Chemistry Serine 03 medical and health sciences 0302 clinical medicine [CHIM.ANAL]Chemical Sciences/Analytical chemistry Biogenic amine Animals [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [INFO.INFO-BT]Computer Science [cs]/Biotechnology ComputingMilieux_MISCELLANEOUS 030304 developmental biology chemistry.chemical_classification 0303 health sciences Oxidase test [CHIM.ORGA]Chemical Sciences/Organic chemistry Temperature Rhodotorula Hydrogen-Ion Concentration [CHIM.ORGA] Chemical Sciences/Organic chemistry Ascorbic acid Rats [SDV.BIO] Life Sciences [q-bio]/Biotechnology 3. Good health Oxygen Microelectrode [INFO.INFO-BT] Computer Science [cs]/Biotechnology chemistry Biochemistry [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Microelectrodes Biosensor 030217 neurology & neurosurgery Cysteine |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2008, 80 (5), pp.1589-1597. ⟨10.1021/ac702230w⟩ Analytical Chemistry, 2008, 80 (5), pp.1589-1597. ⟨10.1021/ac702230w⟩ |
| ISSN: | 0003-2700 1520-6882 |
| DOI: | 10.1021/ac702230w⟩ |
| Popis: | International audience; d-Serine is an endogenous ligand for N-methyl-d-aspartate (NMDA) receptors, and alterations in its concentration have been related to several brain disorders, especially schizophrenia. It is therefore an important target neuromodulator for the pharmaceutical industry. To monitor d-serine levels in vivo, we have developed a microbiosensor based on cylindrical platinum microelectrodes, covered with a membrane of poly-m-phenylenediamine (PPD) and a layer of immobilized d-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). By detecting the hydrogen peroxide produced by enzymatic degradation of d-serine, this microbiosensor shows a detection limit of 16 nM and a mean response time of 2 s. Interferences by ascorbic acid, uric acid, l-cysteine, and by biogenic amines and their metabolites are rejected at more than 97% by the PPD layer. Although several d-amino acids are potential substrates for RgDAAO, d-serine was the only endogenous substrate present in sufficient concentration to be detected by our microbiosensor in the central nervous system. When implanted in the cortex of anesthetized rats, this microbiosensor detected the increase in concentration of d-serine resulting from its diffusion across the blood-brain barrier after an intraperitoneal injection. This new device will make it possible to investigate in vivo the variations in d-serine concentrations occurring under normal and pathological conditions and to assess the pharmacological potency of new drugs designed to impact d-serine metabolism. |
| Databáze: | OpenAIRE |
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