Safety Assessment of Water-Extract Sericin from Silkworm (Bombyx mori) Cocoons Using Different Model Approaches
| Autor: | Yanwu Wang, Siyu Yao, Huafeng Chen, Huiyan Qin, Peng Zhao, Li He, Huili Liang, Guangqiu Qin, Hui Yang, Jiehong Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Article Subject General Immunology and Microbiology Low toxicity biology fungi General Medicine biology.organism_classification medicine.disease_cause Sericin General Biochemistry Genetics and Molecular Biology In vitro 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Bombyx mori In vivo 030220 oncology & carcinogenesis Micronucleus test medicine Medicine Food science Organ weight Genotoxicity |
| Zdroj: | BioMed Research International, Vol 2020 (2020) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Sericin is a natural protein component of silks of silkworm and has potential utility in multiple areas such as pharmacological, cosmetics, and biotechnological industries. However, the understanding of its toxicological safety is still limited. This study evaluated the safety of water-extract sericin from silkworm (Bombyx mori) cocoons using different model approaches, including three genotoxicity studies (the bacterial reverse mutation test, the mammalian erythrocyte micronucleus test, and the mouse spermatogonia chromosomal aberration test) and a 90-day subchronic toxicity study in Sprague-Dawley (SD) rats. The results of this study showed that water-extract sericin was nonmutagenic and nongenotoxic both in vitro and in vivo. Sericin did not induce significant changes in the body and organ weight, food intake, blood hematology and serum biochemistry, urine index, and histopathology in rats. The NOAEL of sericin was determined to be 1 g/kg/day for male and female rats. These results indicated that water-extract sericin was of low toxicity in the experimental conditions of the current study and had the potential for application in food-related products. |
| Databáze: | OpenAIRE |
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