Treatment of contaminated radial fracture in Sprague-Dawley rats by application of a degradable polymer releasing gentamicin
| Autor: | Abraham Nyska, Yossi Lavie, Guy Klaiman, Tal Hagigit, Michal Steiner, Netanel Amouyal, Yuval Ramot, Abraham J. Domb |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class medicine.medical_treatment efficacy Antibiotics Sprague-Dawley (SD) rats Bone healing Toxicology Osteotomy medicine.disease_cause Gastroenterology Pathology and Forensic Medicine biodegradability Internal medicine medicine business.industry animal model Osteomyelitis osteomyelitis medicine.disease fracture Staphylococcus aureus Original Article Gentamicin Histopathology business Cefuroxime medicine.drug |
| Zdroj: | Journal of Toxicologic Pathology |
| ISSN: | 1881-915X 0914-9198 |
| Popis: | Fracture-related infections remain a leading cause of morbidity and mortality. We aimed to establish a simple contaminated radial osteotomy model to assess the efficacy of a biodegradable polymer poly(sebacic-co-ricinoleic acid) [p(SA-RA)] containing 20% w/w gentamicin. A unilateral transverse osteotomy was induced in Sprague-Dawley (SD) rats, followed by application of Staphylococcus aureus suspension over the fracture. After successfully establishing the contaminated open fracture model, we treated the rats either systemically (intraperitoneal cefuroxime), locally with p(SA-RA) containing gentamicin, or both. Control groups included non-contaminated group and contaminated groups that were either untreated or treated with the polymer alone. After 4 weeks, the bones were subjected to micro-CT scanning and microbiological and histopathology evaluations. Micro-CT analysis revealed similar changes in the group subjected to both local and systemic treatment as in the non-contaminated control group. Lack of detectable bacterial growth was noted in most animals of the group subjected to both local and systemic treatment, and all samples were negative for S. aureus. Histopathological evaluation revealed that all treatment modalities containing antibiotics were highly effective in reducing infection and promoting callus repair, resulting in early bone healing. While p(SA-RA) containing gentamicin treatment showed better results than cefuroxime, the combination of local and systemic treatment displayed the highest therapeutic potential in this model. |
| Databáze: | OpenAIRE |
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