Gonadotrophin stimulation of non-luteinized granulosa cells increases steroid production and the expression of enzymes involved in estrogen and progesterone synthesis

Autor: Olli Ritvos, K. Carlström, O. Hovatta, M. Lindeberg
Rok vydání: 2006
Předmět:
endocrine system
medicine.medical_specialty
medicine.drug_class
Granulosa cell
Gene Expression
Chorionic Gonadotropin
03 medical and health sciences
Aromatase
0302 clinical medicine
Internal medicine
medicine
Humans
Cholesterol Side-Chain Cleavage Enzyme
Ovarian follicle
Cells
Cultured
Progesterone
030304 developmental biology
0303 health sciences
Granulosa Cells
030219 obstetrics & reproductive medicine
biology
urogenital system
Rehabilitation
Obstetrics and Gynecology
Estrogens
Receptors
LH
3. Good health
In vitro maturation
medicine.anatomical_structure
Endocrinology
Reproductive Medicine
Estrogen
Cell culture
biology.protein
Receptors
FSH
Female
sense organs
Follicle Stimulating Hormone
Gonadotropin
Follicle-stimulating hormone receptor
hormones
hormone substitutes
and hormone antagonists
Zdroj: Human Reproduction. 22:401-406
ISSN: 1460-2350
0268-1161
Popis: BACKGROUND: In regular IVF treatment, mature oocytes are collected with their luteinized granulosa cells (GCs). When in vitro maturation (IVM) of the oocytes is performed, non-luteinized GCs can be collected. We have investigated how these cells respond to gonadotrophin stimulation in culture. METHODS: GCs were collected from patients undergoing IVM treatment and compared with GCs from IVF patients. The cells were stimulated with FSH and/or hCG. After 48 h, culture media were collected for hormone analysis, and RNA was isolated for gene expression analysis. RESULTS: In IVM GCs, hCG and FSH alone and in combination induced significantly increased progesterone production, and FSH alone and in combination with hCG increased estrogen production. We also studied the gene expression of P-450 aromatase and P-450 scc and the receptors for FSH and LH. In non-luteinized GCs, the expression levels of P-450 aromatase increased with all treatments, and P-450 scc expression increased with the combined FSH and hCG treatment. LHR expression increased with FSH treatment, but the FSH receptor expression did not change with different treatments. CONCLUSIONS: Non-luteinized GCs behaved differently from luteinized GCs in culture. The data help understand the final stages of maturation of human oocytes and follicles.
Databáze: OpenAIRE
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