Transcriptome Analyses Reveal Differential Transcriptional Profiles in Early- and Late-Dividing Porcine Somatic Cell Nuclear Transfer Embryos
| Autor: | Changjiang Xu, Bingyuan Wang, Zhiguo Liu, Kui Li, Guangming Xiang, Yulian Mu, Jingjing Che, Kui Xu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
pig Nuclear Transfer Techniques animal structures lcsh:QH426-470 Cell division Transcription Genetic Somatic cell Swine Cloning Organism Biology somatic cell nuclear transfer Article Transcriptome 03 medical and health sciences 0302 clinical medicine Gene expression Genetics medicine Animals Blastocyst Gene Genetics (clinical) Cell Nucleus 030219 obstetrics & reproductive medicine Gene Expression Profiling embryo development Cellular Reprogramming Cell biology lcsh:Genetics 030104 developmental biology medicine.anatomical_structure embryonic structures gene expression Somatic cell nuclear transfer RNA-seq Reprogramming |
| Zdroj: | Genes Volume 11 Issue 12 Genes, Vol 11, Iss 1499, p 1499 (2020) |
| ISSN: | 2073-4425 |
| Popis: | Somatic cell nuclear transfer (SCNT) is not only a valuable tool for understanding nuclear reprogramming, but it also facilitates the generation of genetically modified animals. However, the development of SCNT embryos has remained an uncontrollable process. It was reported that the SCNT embryos that complete the first cell division sooner are more likely to develop to the blastocyst stage, suggesting their better developmental competence. Therefore, to better understand the underlying molecular mechanisms, RNA-seq of pig SCNT embryos that were early-dividing (24 h postactivation) and late-dividing (36 h postactivation) was performed. Our analysis revealed that early- and late-dividing embryos have distinct RNA profiles, and, in all, 3077 genes were differentially expressed. Gene ontology (GO)and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that early-dividing embryos exhibited higher expression in genes that participated in the meiotic cell cycle, while enrichment of RNA processing- and translation-related genes was found in late-dividing embryos. There are also fewer somatic memory genes such as FLRT2, ADAMTS1, and FOXR1, which are abnormally activated or suppressed in early-dividing cloned embryos. These results show that early-dividing SCNT embryos have different transcriptional profiles than late-dividing embryos. Early division of SCNT embryos may be associated with their better reprogramming capacity, and somatic memory genes may act as a reprogramming barrier in pig SCNT reprogramming. |
| Databáze: | OpenAIRE |
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