Platelets Inhibit Migration of Canine Osteosarcoma Cells
| Autor: | Camilo Bulla, Sandra C Bulla, Kari V. Lunsford, Peres Ramos Badial, R.C. Silva |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Blood Platelets
0301 basic medicine Epithelial-Mesenchymal Transition Biology Polymerase Chain Reaction Canine Osteosarcoma Pathology and Forensic Medicine Metastasis 03 medical and health sciences Dogs Downregulation and upregulation Cell Movement Cell Line Tumor medicine Animals Platelet Platelet activation Osteosarcoma General Veterinary Cell migration medicine.disease Coculture Techniques Disease Models Animal 030104 developmental biology Immunology Disease Progression Cancer research Platelet lysate |
| Zdroj: | Journal of Comparative Pathology. 156:3-13 |
| ISSN: | 0021-9975 |
| DOI: | 10.1016/j.jcpa.2016.10.001 |
| Popis: | The interaction between platelets and tumour cells is important for tumour growth and metastasis. Thrombocytopenia or antiplatelet treatment negatively impact on cancer metastasis, demonstrating potentially important roles for platelets in tumour progression. To our knowledge, there is no information regarding the role of platelets in cancer progression in dogs. This study was designed to test whether canine platelets affected the migratory behaviour of three canine osteosarcoma cell lines and to give insights of molecular mechanisms. Intact platelets, platelet lysate and platelet releasate inhibited the migration of canine osteosarcoma cell lines. Addition of blood leucocytes to the platelet samples did not alter the inhibitory effect on migration. Platelet treatment also significantly downregulated the transcriptional levels of SNAI2 and TWIST1 genes. The interaction between canine platelets or molecules released during platelet activation and these tumour cell lines inhibits their migration, which suggests that canine platelets might antagonize metastasis of canine osteosarcoma. This effect is probably due to, at least in part, downregulation of genes related to epithelial-mesenchymal transition. |
| Databáze: | OpenAIRE |
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