Circulation of bluetongue virus in goats in the Karamoja region of Uganda
| Autor: | Samuel Majalija, Chrisostom Ayebazibwe, C.A.L. Oura, Carrie Batten, Elijah N. Mulabbi |
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| Rok vydání: | 2013 |
| Předmět: |
Veterinary medicine
Prevalence Antibodies Viral Bluetongue Virus Serology Blood serum Seroepidemiologic Studies Animals Seroprevalence Uganda lcsh:Veterinary medicine Goat Diseases General Veterinary biology Goats RNA virus General Medicine biology.organism_classification Virology Reverse transcription polymerase chain reaction Bluetongue virus Goats Karamoja Uganda biology.protein lcsh:SF600-1100 Antibody Bluetongue virus Karamoja |
| Zdroj: | Journal of the South African Veterinary Association, Vol 84, Iss 1, Pp 1-3 (2013) Journal of the South African Veterinary Association, Vol 84, Iss 1, Pp e1-e3 (2013) Journal of the South African Veterinary Association, Volume: 84, Issue: 1, Published: 2013 |
| ISSN: | 2224-9435 1019-9128 |
| DOI: | 10.4102/jsava.v84i1.922 |
| Popis: | The presence of bluetongue virus (BTV) in indigenous goats from the Karamoja region of northern Uganda was investigated. A total of 300 goats were sampled (serum and whole blood) from five districts within the Karamoja region. The samples were analysed for the presence of bluetongue (BT) antibodies using a commercial Enzyme-linked immunosorbent assay (ELISA) and for the presence of BTV viral RNA by real-time Reverse transcription polymerase chain reaction (RT-PCR), because BTV is an RNA virus. Of the 300 goats tested, 269 (90%) were positive for BTV antibodies, indicating high levels of BTV circulation within the region. Out of the 150 whole blood samples tested for the presence of the virus by real-time RT-PCR, 84 (56%) were positive for BTV RNA. This study, which is the first of its kind in Uganda, showed a high seroprevalence of BT antibodies and active circulation of BTV in a high proportion of goats in the Karamoja region. |
| Databáze: | OpenAIRE |
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