Regulation of cytokine-induced neutrophil chemoattractant (CINC) mRNA production in cultured rat cells
| Autor: | Joseph E. Delarco, Gregory J. Dolecki |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Transcription Genetic medicine.medical_treatment Molecular Sequence Data Biology Cell Line chemistry.chemical_compound Genetics medicine Animals Amino Acid Sequence RNA Messenger RNA Processing Post-Transcriptional Growth Substances Molecular Biology Regulation of gene expression Messenger RNA Base Sequence Chemotactic Factors Tumor Necrosis Factor-alpha Interleukin Chemotaxis Cell Biology General Medicine Fibroblasts Molecular biology Rats Cytokine chemistry Gene Expression Regulation Cell culture Culture Media Conditioned Intercellular Signaling Peptides and Proteins Tumor necrosis factor alpha Chemokines CXC Interleukin-1 |
| Zdroj: | DNA and cell biology. 13(9) |
| ISSN: | 1044-5498 |
| Popis: | Rat cytokine-induced neutrophil chemoattractant (CINC) is an 8-kD polypeptide originally purified from media conditioned by interleukin-1 beta (IL-1 beta)-stimulated 52E, an epithelioid clone derived from the normal rat kidney (NRK) cell line. Using a fibroblastic clone of NRK cells, 49F, we found that lipopolysaccharide (LPS), IL-1 beta, and tumor necrosis factor-alpha (TNF-alpha) each induce synthesis of CINC mRNA and CINC, although in qualitatively and quantitatively different patterns. Through deadenylation experiments and by probing with oligonucleotides, we discovered that the smaller of the two major CINC transcripts appears to arise from the larger as a result of poly(A) tail removal and/or 3' cleavage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|