Metabolic Phenotyping Study of Mouse Brains Following Acute or Chronic Exposures to Ethanol
| Autor: | Emily G. Armitage, Ioannis Taitzoglou, Neil Loftus, Helen G. Gika, Ian D. Wilson, Amvrosios Orfanidis, Christina Virgiliou, Olga Deda |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Purine Pharmacology Biochemistry Mass Spectrometry Mice 03 medical and health sciences chemistry.chemical_compound Metabolomics medicine Animals Acetylcarnitine Chromatography High Pressure Liquid chemistry.chemical_classification Ethanol 030102 biochemistry & molecular biology General Chemistry Metabolism Adenosine Amino acid Mice Inbred C57BL 030104 developmental biology chemistry Toxicity Female Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Proteome Research. 19:4071-4081 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/acs.jproteome.0c00440 |
| Popis: | The chronic and acute effect of ethanol administration on the metabolic phenotype of mouse brain was studied in a C57BL/6 mouse model of ethanol abuse using both untargeted and targeted ultra performance liquid chromatography-tandem mass spectrometry. Two experiments based on either chronic (8 week) exposure to ethanol of both male and female mice or acute exposure of male mice for 11 days, plus 2 oral gavage doses of 25% ethanol, were undertaken. Marked differences were found in amino acids, nucleotides, nucleosides, and related metabolites as well as a number of different lipids. Using untargeted metabolite profiling, acute ethanol exposure found significant decreases in several metabolites including nucleosides, fatty acids, glycerophosphocholine, and a number of phospholipids, while chronic exposure resulted in increases in several amino acids with notable decreases in adenosine, acetylcarnitine, and galactosylceramides. Similarly, targeted metabolite analysis, focusing on the hydrophilic fraction of the brain tissue extract, identified significant decreases in the metabolism of amino acids and derivatives, as well as purine degradation especially after chronic exposure to ethanol. |
| Databáze: | OpenAIRE |
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