Estrogen Receptor Alpha mRNA in Human Skeletal Muscles
| Autor: | Francoise Rannou-Bekono, Christophe Tiffoche, P. Granier, Marie-Lise Thieulant, Paul Delamarche, Sophie Lemoine |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Male medicine.medical_specialty DNA Complementary medicine.drug_class Receptor expression Physical Therapy Sports Therapy and Rehabilitation Reference Values Internal medicine Deltoid muscle medicine Humans Orthopedics and Sports Medicine RNA Messenger Muscle Skeletal Electrophoresis Agar Gel Reverse Transcriptase Polymerase Chain Reaction Chemistry Estrogen Receptor alpha Myometrium Skeletal muscle medicine.anatomical_structure Endocrinology Receptors Estrogen Estrogen Agarose gel electrophoresis Female Nested polymerase chain reaction Estrogen receptor alpha |
| Zdroj: | Medicine & Science in Sports & Exercise. 35:439-443 |
| ISSN: | 0195-9131 |
| Popis: | Introduction/purpose To explain the effect of estrogen on skeletal muscle, the presence of estrogen receptor alpha mRNA (ERalpha mRNA) was investigated in human skeletal muscle. Methods The highly sensitive technique of nested reverse transcriptase-polymerase chain reaction (nested RT-PCR) was applied on a variety of tissue samples of both sexes: women (deltoid, pectoral, and uterus muscles) (N= 3) and men (deltoid muscle) (N= 3). The total ribonucleic acid was isolated from each tissue sample, reverse transcribed in a thermocycler, and nested PCR was then performed with specific primers. The by-products were analyzed by agarose gel electrophoresis. Internal standard 28S was simultaneously amplified. The ERalpha mRNA level was quantitated by using the ERalpha mRNA/28S mRNA ratio. Results The expected 204-bp product corresponding to ERalpha was amplified in all tested tissue samples, i.e., deltoid, pectoral, and uterine muscles from women and deltoid muscle from men. The ERalpha mRNA/28S mRNA ratios indicating the receptor expression levels in deltoid muscle from men and women were 0.945 +/- 0.393 (mean +/- SD) (N= 3) and 0.973 +/- 0.136 (mean +/- SD) (N= 2), respectively. Conclusions In conclusion, the nested RT-PCR technique identified the presence of transcript encoding ERalpha mRNA in human skeletal muscles. Semi-quantification did not reveal gender difference. |
| Databáze: | OpenAIRE |
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