Adaptation in toxic environments: comparative genomics of loci carrying antibiotic resistance genes derived from acid mine drainage waters

Autor: Florence Arsène-Ploetze, Olfa Chiboub, Didier Lièvremont, Julien Farasin, Kelle C. Freel, Stephanie Fouteau, Valérie Barbe
Přispěvatelé: Génétique moléculaire, génomique, microbiologie (GMGM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Géosciences Rennes (GR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université de Strasbourg, Centre National de la Recherche Scientifique, ANR-12-ADAP-0013, Agence Nationale de la Recherche, Région Alsace, Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), ANR-12-ADAP-0013,THIOFILM,Rôle des biofilms dans l'adaptation et la variabilité génomique des bactéries du genre Thiomonas, impliqués dans les processus de remédiation naturelle dans les drainages miniers :(2012), Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut de Chimie de Strasbourg, Centre National de la Recherche Scientifique (CNRS)-Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC), Laboratoire de Chimie Quantique, Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), univOAK, Archive ouverte, Thiriet, Lydie
Rok vydání: 2017
Předmět:
0301 basic medicine
Health
Toxicology and Mutagenesis
030106 microbiology
Adaptation
Biological
Wastewater
[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
complex mixtures
Mining
03 medical and health sciences
Metals
Heavy
Databases
Genetic
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Environmental Chemistry
[SDV.BV] Life Sciences [q-bio]/Vegetal Biology
Extrusion pumps
ComputingMilieux_MISCELLANEOUS
[SDV.GEN]Life Sciences [q-bio]/Genetics
Bacteria
Comparative genomics
Acid mine drainage (AMD)
Drug Resistance
Microbial
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Genomics
Horizontal gene transfer
General Medicine
equipment and supplies
Pollution
Anti-Bacterial Agents
Genome evolution
030104 developmental biology
bacteria
Acids
Zdroj: Environmental Science and Pollution Research
Environmental Science and Pollution Research, Springer Verlag, 2018, 25 (2), pp.1470-1483. ⟨10.1007/s11356-017-0535-8⟩
Environmental Science and Pollution Research, 2018, 25 (2), pp.1470-1483. ⟨10.1007/s11356-017-0535-8⟩
Environmental Science and Pollution Research, Springer Verlag, 2018, 25 (2), pp.1484-1485
Environmental Science and Pollution Research, Springer Verlag, 2018
ISSN: 1614-7499
0944-1344
DOI: 10.1007/s11356-017-0535-8
Popis: International audience; Several studies have suggested the existence of a close relationship between antibiotic-resistant phenotypes and resistance to other toxic compounds such as heavy metals, which involve co-resistance or cross-resistance mechanisms. A metagenomic library was previously constructed in Escherichia coli with DNA extracted from the bacterial community inhabiting an acid mine drainage (AMD) site highly contaminated with heavy metals. Here, we conducted a search for genes involved in antibiotic resistance using this previously constructed library. In particular, resistance to antibiotics was observed among five clones carrying four different loci originating from CARN5 and CARN2, two genomes reconstructed from the metagenomic data. Among the three CARN2 loci, two carry genes homologous to those previously proposed to be involved in antibiotic resistance. The third CARN2 locus carries a gene encoding a membrane transporter with an unknown function and was found to confer bacterial resistance to rifampicin, gentamycin, and kanamycin. The genome of Thiomonas delicata DSM 16361 and Thiomonas sp. X19 were sequenced in this study. Homologs of genes carried on these three CARN2 loci were found in these genomes, two of these loci were found in genomic islands. Together, these findings confirm that AMD environments contaminated with several toxic metals also constitute habitats for bacteria that function as reservoirs for antibiotic resistance genes.
Databáze: OpenAIRE
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