EPA attenuates ultraviolet radiation-induced downregulation of aquaporin-3 in human keratinocytes

Autor: Ho Sub Lee, Kun-Kuk Lee, Ghang-Tai Lee, Yeun-Ja Mun, Won-Hong Woo, Moon-Kyung Kang, Byoung-Kook Jeon
Rok vydání: 2014
Předmět:
Zdroj: Archives of Pharmacal Research. 38:1552-1560
ISSN: 1976-3786
0253-6269
DOI: 10.1007/s12272-014-0482-6
Popis: Eicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid (ω-3 PUFA) that protects against photodamage and photocarcinogenesis in mammals. Aquaporin-3 (AQP3) is a water/glycerol transport protein that is found in basal layer keratinocytes. In this study, we have investigated the protective effect of EPA against ultraviolet B (UVB)-induced AQP3 downregulation in human keratinocytes. EPA treatment was found to increase AQP3 gene and protein expression in human epidermal keratinocytes (HaCaT). Using a specific inhibitor, we observed that the effect of EPA on AQP3 expression was mediated by extracellular signal-regulated kinase (ERK) activation. UVB radiation induced AQP3 downregulation in HaCaT cells, and it was found that EPA treatment attenuated UVB-induced AQP3 reduction and the associated cell death. UVB-induced downregulation of AQP3 was blocked by EPA and p38 inhibitor SB203580. Collectively, the present results show that EPA increased AQP3 expression and that this led to a reduction UVB-induced photodamage.
Databáze: OpenAIRE
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