Magnetic resonance imaging of transplanted neural stem cells in Parkinson disease rats
| Autor: | Xian-li Zhu, Jiashan Zhao, Lin Yang, Ying Xia, Hongyang Zhao |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Biomedical Engineering Contrast Media Biochemistry Biomaterials Rats Sprague-Dawley In vivo Neurosphere Genetics medicine Animals Neurons medicine.diagnostic_test business.industry Chemistry Magnetic resonance imaging Parkinson Disease Signal on Magnetic Resonance Imaging Neural stem cell Rats Transplantation nervous system Lipofectamine Nuclear medicine business Superparamagnetic iron oxide Stem Cell Transplantation |
| Zdroj: | Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 26(4) |
| ISSN: | 1672-0733 |
| Popis: | In this study we implanted magnetically labeled neural stem cells (NSCs) in PD rats and then monitored their survival and migration in the host brain by magnetic resonance imaging (MRI). The mesencephalic NSCs were obtained from the brain of SD rats. Superparamagnetic iron oxide (SPIO) was transferred to NSCs by Lipofectamine transfection. Eighteen PD lesioned rats were selected for transplantation by evaluation of their rotational behavior in response to amphetamine and randomly assigned to 3 groups, i.e., sham group, PBS group and NSCs transplanted group, with 6 rats in each group. MR scanning was performed at 1, 2, 4, 6, 8 and 10 week(s) following transplantation. At the meantime, rotational behavior was assessed in each group. Our results showed that SPIO particles were clearly visible with Prissian blue staining in neurospheres and cells derived from NSCs. The rotational behavior of the NSCs transplanted group was remarkably improved compared with that of sham group and PBS group (P |
| Databáze: | OpenAIRE |
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