Electrochemical characterization of the artificial metalloenzyme papain-[(η6-arene)Ru(1,10-phenanthroline)Cl]+
| Autor: | Jakub Šebera, Štěpánka Nováková Lachmanová, Barisa Talbi, Magdaléna Hromadová, Michèle Salmain, Lubomír Pospíšil |
|---|---|
| Přispěvatelé: | J. Heyrovský Institute of Physical Chemistry of the ASCR, Czech Academy of Sciences [Prague] (CAS), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL), Institut Parisien de Chimie Moléculaire (IPCM), Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Chemical Biology (CHEMBIO), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Chimie Moléculaire de Paris Centre (FR 2769) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
[CHIM.ORGA]Chemical Sciences/Organic chemistry
General Chemical Engineering Phenanthroline 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Transfer hydrogenation Electrochemistry 01 natural sciences Combinatorial chemistry 3. Good health 0104 chemical sciences Analytical Chemistry Dielectric spectroscopy Catalysis Papain chemistry.chemical_compound chemistry [CHIM.COOR]Chemical Sciences/Coordination chemistry 0210 nano-technology Voltammetry ComputingMilieux_MISCELLANEOUS Conjugate |
| Zdroj: | Journal of Electroanalytical Chemistry Journal of Electroanalytical Chemistry, Elsevier 2020, 859, pp.113882. ⟨10.1016/j.jelechem.2020.113882⟩ |
| ISSN: | 1572-6657 |
| DOI: | 10.1016/j.jelechem.2020.113882⟩ |
| Popis: | Electrochemical properties were studied for [(η6-arene)Ru(1,10-phenanthroline)Cl]Cl (arene = C6H5(CH2)2NHCOCH2Cl) organometallic complex 1, protein Papain PAP and its conjugate with organometallic complex 1-PAP. The latter can serve as an artificial metalloenzyme with catalytic activity in transfer hydrogenation. This work demonstrates that AC voltammetry and electrochemical impedance spectroscopy can be used as fast tools to screen the catalytic ability of 1-PAP electrochemically by studies of the catalytic hydrogen evolution reaction (HER). Proteins are known to catalyze this process, but we have shown that additional HER signal associated with the catalytic activity of 1 is observed for its conjugate with Papain 1-PAP. |
| Databáze: | OpenAIRE |
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