Nociceptors Lacking TRPV1 and TRPV2 Have Normal Heat Responses
| Autor: | Brian M. Davis, Kathryn M. Albers, H. Richard Koerber, Michael J. Caterina, Martin Koltzenburg, Melissa Zwick, C. Jeffery Woodbury, Jeffrey J. Lawson, Shuying Wang |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Hot Temperature Receptors Drug Molecular Sequence Data TRPV2 TRPV1 TRPV Cation Channels Ion Channels Rats Sprague-Dawley Mice chemistry.chemical_compound Species Specificity Ganglia Spinal medicine Animals Amino Acid Sequence Neurons Afferent Receptor Mice Inbred BALB C Mice Inbred C3H musculoskeletal neural and ocular physiology General Neuroscience Nociceptors Rats Cell biology Nociception nervous system chemistry Capsaicin Hyperalgesia Nociceptor Female lipids (amino acids peptides and proteins) Calcium Channels Epidermis Plant Lectins medicine.symptom Brief Communications Neuroscience psychological phenomena and processes Intracellular |
| Zdroj: | The Journal of Neuroscience. 24:6410-6415 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | Vanilloid receptor 1 (TRPV1) has been proposed to be the principal heat-responsive channel for nociceptive neurons. The skin of both rat and mouse receives major projections from primary sensory afferents that bind the plant lectin isolectin B4 (IB4). The majority of IB4-positive neurons are known to be heat-responsive nociceptors. Previous studies suggested that, unlike rat, mouse IB4-positive cutaneous afferents did not express TRPV1 immunoreactivity. Here, multiple antisera were used to confirm that mouse and rat have different distributions of TRPV1 and that TRPV1 immunoreactivity is absent in heat-sensitive nociceptors. Intracellular recording in TRPV1-/-mice was then used to confirm that TRPV1 was not required for detecting noxious heat. TRPV1-/-mice had more heat-sensitive neurons, and these neurons had normal temperature thresholds and response properties. Moreover, in TRPV1-/-mice, 82% of heat-responsive neurons did not express immunoreactivity for TRPV2, another putative noxious heat channel. |
| Databáze: | OpenAIRE |
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