Various dictyostelids from the environment can produce multilamellar bodies
| Autor: | Steve J. Charette, Alicia F. Durocher, Cynthia Gagné-Thivierge |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
biology 030306 microbiology Chemistry Immunology macromolecular substances General Medicine Environment biology.organism_classification Applied Microbiology and Biotechnology Microbiology Dictyostelium Cellular Structures 03 medical and health sciences Membrane Phenotype Genetics Biophysics Protozoa Molecular Biology 030304 developmental biology |
| Zdroj: | Canadian journal of microbiology. 66(12) |
| ISSN: | 1480-3275 |
| Popis: | Multilamellar bodies (MLBs), structures composed of concentric membrane layers, are known to be produced by different protozoa, including species of ciliates, free-living amoebae, and Dictyostelium discoideum social amoebae. Initially believed to be metabolic waste, potential roles like cell communication and food storage have been suggested for D. discoideum MLBs, which could be useful for the multicellular development of social amoebae and as a food source. However, among dictyostelids, this phenomenon has only been observed with D. discoideum, and mainly with laboratory strains grown in axenic conditions. It was thought that other social amoebae may also produce MLBs. Four environmental social amoeba isolates were characterized. All strains belong to the Dictyostelium genus, including some likely to be Dictyostelium giganteum. They have distinctive phenotypes comprising their growth rate on Klebsiella aerogenes lawns and the morphology of their fruiting bodies. They all produce MLBs like those produced by a D. discoideum laboratory strain when grown on K. aerogenes lawns, as revealed by analysis using the H36 antibody in epifluorescence microscopy as well as by transmission electron microscopy. Consequently, this study shows that MLBs are produced by various dictyostelid species, which further supports a role for MLBs in the lifestyle of amoebae. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|