Extracellular Matrix Oxidised by the Granulocyte Oxidants Hypochlorous and Hypobromous Acid Reduces Lung Fibroblast Adhesion and Proliferation In Vitro
Autor: | David M. van Reyk, Dikaia Xenaki, Patrick He, Brian G. Oliver, Sandra Rutting, Michael Papanicolaou, Alaina J. Ammit |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
QH301-705.5
Granulocyte medicine.disease_cause Article Collagen Type I Extracellular matrix Pathogenesis Fibrosis medicine Cell Adhesion Humans Biology (General) Fibroblast Cell Shape Lung Cell Proliferation ECM Chemistry Bromates fibrosis General Medicine respiratory system Fibroblasts Middle Aged medicine.disease HOX Oxidants Cell biology Extracellular Matrix Hypochlorous Acid medicine.anatomical_structure Cytokines Cytokine secretion Inflammation Mediators Oxidation-Reduction Oxidative stress Granulocytes |
Zdroj: | Cells Cells; Volume 10; Issue 12; Pages: 3351 Cells, Vol 10, Iss 3351, p 3351 (2021) |
ISSN: | 2073-4409 |
Popis: | Chronic airway inflammation and oxidative stress play crucial roles in the pathogenesis of chronic inflammatory lung diseases, with airway inflammation being a key driving mechanism of oxidative stress in the lungs. Inflammatory responses in the lungs activate neutrophils and/or eosinophils, leading to the generation of hypohalous acids (HOX). These HOX oxidants can damage the extracellular matrix (ECM) structure and may influence cell–ECM interactions. The ECM of the lung provides structural, mechanical, and biochemical support for cells and determines the airway structure. One of the critical cells in chronic respiratory disease is the fibroblast. Thus, we hypothesised that primary human lung fibroblasts (PHLF) exposed to an oxidised cell-derived ECM will result in functional changes to the PHLF. Here, we show that PHLF adhesion, proliferation, and inflammatory cytokine secretion is affected by exposure to HOX-induced oxidisation of the cell-derived ECM. Furthermore, we investigated the impact on fibroblast function from the presence of haloamines in the ECM. Haloamines are chemical by-products of HOX and, like the HOX, haloamines can also modify the ECM. In conclusion, this study revealed that oxidising the cell-derived ECM might contribute to functional changes in PHLF, a key mechanism behind the pathogenesis of inflammatory lung diseases. |
Databáze: | OpenAIRE |
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