Collagen Content Is Significantly Lower in Restenotic Versus Nonrestenotic Vessels After Balloon Angioplasty in the Atherosclerotic Rabbit Model
| Autor: | William D. Coats, Peter Whittaker, David T. Cheung, David P. Faxon, Bo Han, Jesse W. Currier |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Pathology medicine.medical_specialty Intimal hyperplasia Arteriosclerosis medicine.medical_treatment Iliac Artery Muscle Smooth Vascular Extracellular matrix Hydroxyproline chemistry.chemical_compound Restenosis Recurrence Physiology (medical) Angioplasty medicine Animals Gelatinase Collagenases business.industry Metalloendopeptidases medicine.disease chemistry Gelatinases Collagenase Matrix Metalloproteinase 2 Regression Analysis Collagen Rabbits Matrix Metalloproteinase 1 Cardiology and Cardiovascular Medicine business Angioplasty Balloon Biomarkers medicine.drug |
| Zdroj: | Circulation. 95:1293-1300 |
| ISSN: | 1524-4539 0009-7322 |
| Popis: | Background It is recognized that restenosis is primarily due to alterations in geometric remodeling of the extracellular matrix rather than intimal hyperplasia. Prior studies have shown that angioplasty stimulates an increase in both synthesis and degradation of collagen in the atherosclerotic vessel. However, differences in collagen content and metabolism between restenotic and nonrestenotic vessels have not been examined. Methods and Results Four weeks after angioplasty in an atherosclerotic rabbit model, collagen content in restenotic and nonrestenotic vessels was measured both biochemically by hydroxyproline quantitation and histologically by a digital subtraction method with the use of circularly polarized images of picrosirius red–stained sections. Collagenase and gelatinase activity also were measured in the same restenotic and nonrestenotic vessels by use of a radiosubstrate assay. Collagen content was found to be significantly lower in restenotic vessels than in nonrestenotic vessels both biochemically (127.0±32.6 versus 212.6±84.3 μg/mg tissue; n=11 vessels; P P =.05). There was a significant inverse correlation between biochemically determined collagen content and gelatinase activity ( P =.02) and a significant correlation between histologically determined lumen area and percent collagen content ( P =.0071). Conclusions Collagen content is significantly decreased in restenotic versus nonrestenotic vessels after angioplasty in the atherosclerotic rabbit model. The increased collagen content in nonrestenotic vessels was associated with preserved lumen area and may play a role in geometric remodeling after angioplasty. |
| Databáze: | OpenAIRE |
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