Specific initiation of transcription at a cyanobacterial promoter with RNA polymerase purified from Calothrix sp. PCC 7601
| Autor: | Ghislain Schyns, Nicole Tandeau de Marsac, Jean Houmard, André Sobczyk |
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| Rok vydání: | 1994 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Sigma Factor Biology Cyanobacteria Microbiology chemistry.chemical_compound Bacterial Proteins Gene Expression Regulation Plant Sigma factor Transcription (biology) RNA polymerase Operon Gene expression Promoter Regions Genetic Molecular Biology RNA polymerase II holoenzyme Gene Plant Proteins Base Sequence Phycocyanin Promoter DNA-Directed RNA Polymerases Gene Expression Regulation Bacterial Molecular biology chemistry bacteria Transcription factor II D |
| Zdroj: | Molecular Microbiology. 13:887-896 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1994.tb00480.x |
| Popis: | Summary Although in cyanobacteria many genes have been shown to be transcriptionally controlled by specific stimuli, little is known about promoter structure and the form of RNA polymerase that recognizes individual promoters. RNA polymerase holoenzyme has been purified from Calothrix sp. PCC 7601. its polypeptide composition resembles that of the plant chloroplast enzymes. To study transcription in cyanobacteria further, we have analysed the promoter-recognition properties of the purified enzyme. In vitro transcription was assayed with the promoter of the phycocyanin gene (cpc1) that is expressed whatever the incident light conditions. Transcription initiation at the same start point as in vivo was obtained with the Calothrix sp. PCC 7601 purified enzyme and the Escherichia coli core enzyme supplemented with a Calothrix sp. PCC 7601 sigma factor, but not with the E. coli holoenzyme. |
| Databáze: | OpenAIRE |
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