Biochemical markers of the mechanical quality of engineered hyaline cartilage
| Autor: | Daniel J. Kelly, Paul V. Hatton, Sally C. Dickinson, Jenny Mundy, Patrick J. Prendergast, Aileen Crawford, T J Sims, Anthony P. Hollander |
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| Rok vydání: | 2007 |
| Předmět: |
Scaffold
Materials science Statistics as Topic Biomedical Engineering Biophysics Aggregate modulus Bioengineering Biomaterials Glycosaminoglycan Extracellular matrix Hydroxyproline chemistry.chemical_compound Chondrocytes Tissue engineering Materials Testing medicine Animals GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Cells Cultured Biochemical markers Glycosaminoglycans Tissue Engineering Hyaline cartilage Cell Differentiation Administrative Staff Authors Elasticity Hyaline Cartilage medicine.anatomical_structure chemistry Cattle Collagen Stress Mechanical Biomarkers Biomedical engineering |
| Zdroj: | Journal of Materials Science: Materials in Medicine. 18:273-281 |
| ISSN: | 1573-4838 0957-4530 |
| DOI: | 10.1007/s10856-006-0689-2 |
| Popis: | PUBLISHED The aim of this study was to determine whether or not biochemical markers can be used as surrogate measures for the mechanical quality of tissue engineered cartilage. The biochemical composition of tissue engineered cartilage constructs were altered by varying either (i) the initial cell seeding density of the scaffold (seeding density protocol) or (ii) the length of time the engineered tissue was cultured (culture period protocol). The aggregate or Young?s moduli of the constructs were measured (by confined or unconfined compression respectively), and compared with the composition of the extracellular matrix by quantitative measurement of the glycosaminoglycan (GAG), hydroxyproline, collagen I and collagen II and collagen cross-links. The aggregate modulus correlated positively with both GAG and collagen II content, but not with collagen I content. Young?s modulus correlated positively with GAG, collagen II and collagen I content, and the ratio of mature to immature cross-links, but not with hydroxyproline content. These results suggested that hydroxyproline may be an unreliable indicator of mechanical quality of tissue engineered cartilage, and that a measure of collagen II and GAG content is required to predict the biomechanical quality of tissue engineered cartilage. This work was funded by the European Union (EU) under the SCAFCART project (contract G5RD-CT-1999-00050). APH is funded in part by an endowed chair from the UK Arthritis Research Campaign. |
| Databáze: | OpenAIRE |
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