Effects of Hyperlipoproteinemic Serum and Exogenous Proline Concentration on Collagen Synthesis by Isolated Rabbit Aortas

Autor:	D. Holderbaum, L. A. Ehrhart, K. G. McCullagh
Rok vydání:	1975
Předmět:	Male Proline Chemistry Rabbit aorta Hyperlipidemias Rabbit (nuclear engineering) In Vitro Techniques General Biochemistry Genetics and Molecular Biology In vitro Lipoproteins LDL Biochemistry Animals Collagen Rabbits Incubation Flux (metabolism) Aorta Serum cholesterol
Zdroj:	Experimental Biology and Medicine. 150:363-367
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-150-39036
Popis:	SummaryCollagen synthesis was measured in segments of normal rabbit aorta, incubated in vitro, by monitoring the formation of peptidyl-14C-hydroxyproline from [U-14C]-L-proline added to the incubation medium. The effect of hyperlipoproteinemic rabbit serum on the rate of collagen synthesis was compared with the effect of normal rabbit serum. No differences in the rates of synthesis were detected between the two batches of serum, despite a 60-fold difference in serum cholesterol concentration.Increases in free proline concentration in the incubation medium resulted in changes in proline flux between medium and tissue pools of free proline, but medium proline concentration had no effect on the rate of collagen synthesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd122ab9bb413d11b17ff1f0fb0226e5 https://doi.org/10.3181/00379727-150-39036 Zobrazit plný text záznamu