Improved latex agglutination test for detection of antibodies in serum and cerebrospinal fluid of Trypanosoma brucei gambiense infected patients
| Autor: | N. Van Meirvenne, Veerle Lejon, Philippe Büscher, E. Magnus |
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| Rok vydání: | 1999 |
| Předmět: |
Veterinary (miscellaneous)
Trypanosoma brucei gambiense Antibodies Protozoan Antigens Protozoan Serology Cerebrospinal fluid Antigen medicine Animals Humans Serologic Tests biology medicine.disease Virology Latex fixation test Infectious Diseases Trypanosomiasis African Insect Science biology.protein Parasitology Antibody Trypanosomiasis Serum dilution Latex Fixation Tests Variant Surface Glycoproteins Trypanosoma |
| Zdroj: | Acta tropica. 73(1) |
| ISSN: | 0001-706X |
| Popis: | A rapid latex agglutination test (LATEX/T. b. gambiense) for detection of antibodies in patients infected with Trypanosoma brucei gambiense is presented. The reagent is coated with a mixture of three variable surface antigens of bloodstream form trypanosomes. Two hundred and forty sera and 79 CSF samples from patients with parasitologically confirmed trypanosome infection along with 173 sera and 38 CSF samples from non-trypanosomiasis patients have been tested. At 1:16 serum dilution, test specificity was 99%, while sensitivity ranged from 83.8 to 100% depending on the geographical origin of the samples. Undiluted CSF samples from non-trypanosomiasis and from first stage patients scored negative while 42 out of 66 CSF samples from second stage patients were positive. Stability and reproducibility of the lyophilized reagent were excellent. |
| Databáze: | OpenAIRE |
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