Role of Histone Tails and Single Strand DNA Breaks in Nucleosomal Arrest of RNA Polymerase

Autor:	Nadezhda S. Gerasimova, Nikolay A. Pestov, Vasily M. Studitsky
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	chromatin structure Organic Chemistry nucleosome transcription-coupled DNA repair General Medicine Catalysis Computer Science Applications Inorganic Chemistry DNA loop DNA damage single-strand DNA breaks Physical and Theoretical Chemistry Molecular Biology Spectroscopy
Zdroj:	International Journal of Molecular Sciences Volume 24 Issue 3 Pages: 2295
ISSN:	1422-0067
DOI:	10.3390/ijms24032295
Popis:	Transcription through nucleosomes by RNA polymerases (RNAP) is accompanied by formation of small intranucleosomal DNA loops (i-loops). The i-loops form more efficiently in the presence of single-strand breaks or gaps in a non-template DNA strand (NT-SSBs) and induce arrest of transcribing RNAP, thus allowing detection of NT-SSBs by the enzyme. Here we examined the role of histone tails and extranucleosomal NT-SSBs in i-loop formation and arrest of RNAP during transcription of promoter-proximal region of nucleosomal DNA. NT-SSBs present in linker DNA induce arrest of RNAP +1 to +15 bp in the nucleosome, suggesting formation of the i-loops; the arrest is more efficient in the presence of the histone tails. Consistently, DNA footprinting reveals formation of an i-loop after stalling RNAP at the position +2 and backtracking to position +1. The data suggest that histone tails and NT-SSBs present in linker DNA strongly facilitate formation of the i-loops during transcription through the promoter-proximal region of nucleosomal DNA.
Databáze:	OpenAIRE
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