Non-invasive assessment of animal exercise stress: real-time PCR of GLUT4, COX2, SOD1 and HSP70 in avalanche military dog saliva
| Autor: | Gabriella Guelfi, Silvana Diverio, M. M. Santoro, M. G. Egidi, W. Di Mari, Olimpia Barbato |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Saliva SOD1 Real-Time Polymerase Chain Reaction SF1-1100 Andrology Superoxide dismutase Random Allocation Dogs Superoxide Dismutase-1 Downregulation and upregulation Physical Conditioning Animal Animals Medicine HSP70 Heat-Shock Proteins RNA Messenger HSP70 Glucose Transporter Type 4 biology Superoxide Dismutase business.industry GLUT4 COX2 SOD1 HSP70 dog exercise stress Glucose transporter Avalanches Up-Regulation Hsp70 Animal culture Oxidative Stress Military Personnel Real-time polymerase chain reaction Cyclooxygenase 2 dog exercise stress biology.protein Animal Science and Zoology Energy Metabolism business GLUT4 Biomarkers COX2 |
| Zdroj: | Animal, Vol 9, Iss 1, Pp 104-109 (2015) |
| ISSN: | 1751-7311 |
| Popis: | Exercise has been shown to increase mRNA expression of a growing number of genes. The aim of this study was to assess if mRNA expression of the metabolism- and oxidative stress-related genes GLUT4 (glucose transporter 4), COX2 (cyclooxygenase 2), SOD1 (superoxide dismutase 1) and HSP70 (heat shock protein 70) in saliva changes following acute exercise stress in dogs. For this purpose, 12 avalanche dogs of the Italian Military Force Guardia di Finanza were monitored during simulation of a search for a buried person in an artificial avalanche area. Rectal temperature (RT) and saliva samples were collected the day before the trial (T0), immediately after the descent from a helicopter at the onset of a simulated avalanche search and rescue operation (T1), after the discovery of the buried person (T2) and 2 h later (T3). Expressions of GLUT4, SOD1, COX2 and HSP70 were measured by real-time PCR. The simulated avalanche search and rescue operation was shown to exert a significant effect on RT, as well as on the expression of all metabolism- and oxidative stress-related genes investigated, which peaked at T2. The observed expression patterns indicate an acute exercise stress-induced upregulation, as confirmed by the reductions in expression at T3. Moreover, our findings indicate that saliva is useful for assessing metabolism- and oxidative stress-related genes without the need for restraint, which could affect working dog performance. |
| Databáze: | OpenAIRE |
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