Isolation and chemical analysis of a fatty acid fraction of Kalanchoe pinnata with a potent lymphocyte suppressive activity
| Autor: | V. L. G. da Moraes, A. P. Almeida, L. M. T. R. Lima, Sônia Soares Costa, Bartira Rossi-Bergmann, S. A. G. da Silva, M. L. M. Souza |
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| Rok vydání: | 2000 |
| Předmět: |
T-Lymphocytes
Pharmaceutical Science Lymphocyte proliferation Pharmacognosy Analytical Chemistry Palmitic acid chemistry.chemical_compound Mice In vivo Drug Discovery Animals Pharmacology chemistry.chemical_classification Mice Inbred BALB C Plants Medicinal biology Plant Extracts Organic Chemistry Fatty Acids Fatty acid Kalanchoe biology.organism_classification Complementary and alternative medicine chemistry Biochemistry Saturated fatty acid Molecular Medicine lipids (amino acids peptides and proteins) Stearic acid Cell Division Immunosuppressive Agents |
| Zdroj: | Planta medica. 66(2) |
| ISSN: | 0032-0943 |
| Popis: | Previously we demonstrated that Kalanchoe pinnata (KP) leaf extracts inhibited in vitro lymphocyte proliferation and showed in vivo immunosuppressive activity. Here we attempt to identify the immunosuppressive substances present in KP guided by the lymphoproliferative assays. From the ethanolic extract was purified a fraction (KP12SA) twenty-fold more potent to block murine lymphocyte proliferation than the crude extract. Chemical analysis by 1H- and 13C-NMR, IR and GC-MS of KP12SA (methylated sample) showed 89.3% of palmitic acid (C16), 10.7% of stearic acid (C18) and traces of arachidic (C20) and behenic acids (C22). This study provides evidence that fatty acids present in Kalanchoe pinnata may be responsible, at least in part, for its immunosuppressive effect in vivo. |
| Databáze: | OpenAIRE |
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