Neuronal marker recovery after Simvastatin treatment in dementia in the rat brain: In vivo magnetic resonance study
| Autor: | Jarmila Kucharská, Svatava Kašparová, Tibor Liptaj, Michal Dubovický, O Vancova, Boris Lipták, O Uličná, Siegfried Trattnig, Pavol Szomolanyi, Radka Tušková, Zuzana Sumbalová |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Simvastatin medicine.medical_specialty Magnetic Resonance Spectroscopy Ubiquinone Thiobarbituric acid Spatial Learning Morris water navigation task medicine.disease_cause Thiobarbituric Acid Reactive Substances Behavioral Neuroscience chemistry.chemical_compound Internal medicine medicine TBARS Animals Rats Wistar Nootropic Agents Spatial Memory Aspartic Acid biology Chemistry Brain Galactose Phosphorus Isotopes Dipeptides Organ Size Disease Models Animal Treatment Outcome Endocrinology Biochemistry HMG-CoA reductase biology.protein Dementia Creatine kinase Protons Biomarkers Inositol Oxidative stress medicine.drug |
| Zdroj: | Behavioural Brain Research. 284:257-264 |
| ISSN: | 0166-4328 |
| Popis: | The aim of study was to search for new biomarkers with a magnetic resonance technique to identify the early stages of dementia, induced by d -galactose, and evaluate Simvastatin therapy. Localized proton magnetic resonance spectroscopy measurements showed a significant decrease in the concentration of N-acetylaspartate + N-acetylaspartylglutamate and myo-inositol in the d -galactose group compared to the control group, and, conversely, an increase of N-acetylaspartate + N-acetylaspartylglutamate in the d -galactose/Simvastatin group. Using a saturation transfer experiment, with phosphorus magnetic resonance spectroscopy, we observed a significant elevation of the forward rate constant of the creatine kinase reaction in the brains of the d -galactose group compared to controls, and subsequently, a significant reduction of this reaction in the d -galactose/Simvastatin group. Spatial learning and memory were evaluated using the modified Morris water maze test. The dynamics of the learning process represented by the learning index revealed a significant reduction in learning in the d -galactose group, but the deficits as a consequence of the d -galactose effects were recovered in the d -galactose/Simvastatin group, in which the learning dynamics resembled those of the control group. By determining the thiobarbituric acid reactive substances and total coenzyme Q 9 in plasma, we have shown that long-term administration of d -galactose created conditions for oxidative stress, and that the administration of Simvastatin decreased oxidative stress in plasma. Volumetry analyses from the hippocampal area show a reduction in the segmented area in the d -galactose group, compared with the control group, and an enlarged area in the hippocampus in the d -galactose/Simvastatin group. |
| Databáze: | OpenAIRE |
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