lncRNA expression profiles related to apoptosis and autophagy in peripheral blood mononuclear cells of patients with rheumatoid arthritis

Autor: Jie Wang, Ling Xin, Yanqiu Sun, Hui Jiang, Xinlei Du, Pingheng Zhang, Jian Liu, Xin Wang, Yue Sun, Jianting Wen, Ying Zhang, Lei Wan
Rok vydání: 2020
Předmět:
Zdroj: FEBS Open Bio, Vol 10, Iss 8, Pp 1642-1654 (2020)
FEBS Open Bio
ISSN: 2211-5463
DOI: 10.1002/2211-5463.12913
Popis: Long noncoding RNAs (lncRNAs) are >200‐bp molecules that do not generally code for proteins. Human lncRNAs have well‐characterized roles in gene expression regulation, particularly with regard to protein‐coding genes, and their dysregulation has been linked to disease. Here, we set out to investigate changes in the expression of lncRNAs related to apoptosis and autophagy in the peripheral blood mononuclear cells (PBMCs) of rheumatoid arthritis (RA). In addition, we aimed to correlate lncRNA expression profiles with clinical indexes and self‐perception of patients (SPP). To this end, we employed RNA sequencing of lncRNAs in PBMCs from three patients with RA and three healthy controls. We used bioinformatics to screen several dysregulated lncRNAs related to apoptosis and autophagy. To validate key lncRNA candidates, we performed quantitative reverse transcriptase–PCR on 20 patients with RA and 20 healthy controls. We found the expression of seven lncRNAs (MAPKAPK5‐AS1, ENST00000619282, C5orf17, LINC01189, LINC01006, DSCR9 and MIR22HG) was significantly altered in PBMCs of patients with RA. Receiver operating characteristic curve analysis suggested that MIR22HG [area under the curve (AUC) = 0.846, P = 0.000], DSCR9 (AUC = 0.783, P = 0.005), LINC01189 (AUC = 0.677, P = 0.034), MAPKAPK5‐AS1 (AUC = 0.644, P = 0.025) and ENST00000619282 (AUC = 0.636, P = 0.043) are potential biomarkers of RA. Spearman's correlation analysis revealed selected lncRNAs correlated with clinical indexes and SPP. Therefore, we highlight that some lncRNAs related to apoptosis and autophagy may serve as potential biomarkers for diagnosis and monitoring of RA progression, which also correlate with several clinical indexes and SPP.
We investigated changes in the expression of lncRNAs related to apoptosis and autophagy in the peripheral blood mononuclear cells (PBMCs) of rheumatoid arthritis (RA). Expression of seven lncRNAs (MAPKAPK5‐AS1, ENST00000619282, C5orf17, LINC01189, LINC01006, DSCR9, and MIR22HG) was significantly altered in PBMCs of RA patients. Squares, triangles, and circles represent lncRNAs, miRNAs, and mRNAs, respectively. Red nodes: upregulation; blue nodes: downregulation.
Databáze: OpenAIRE
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