In vitro evaluation of poly[bis(ethyl alanato)phosphazene] as a scaffold for bone tissue engineering
| Autor: | S. Lora, Maria Teresa Conconi, Paolo Carampin, Anna Michela Menti, Pier Paolo Parnigotto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Calcium Phosphates
Scaffold Polymers Osteocalcin Biocompatible Materials Bone and Bones Rats Sprague-Dawley chemistry.chemical_compound Tissue culture Organophosphorus Compounds MICROSPHERES REGENERATION Animals Cell adhesion Cells Cultured Phosphazene POLYMERIC BIOMATERIALS RELEASE Osteoblasts Tissue Engineering Chemistry Cell growth PROGRESSIVE DEVELOPMENT General Engineering PROLIFERATION Substrate (chemistry) PROGRESSIVE DEVELOPMENT POLYMERIC BIOMATERIALS OSTEOBLAST PHENOTYPE DIFFERENTIATION CELLS PROLIFERATION REGENERATION RELEASE MICROSPHERES MATRIX Adhesion Alkaline Phosphatase Rats DIFFERENTIATION Evaluation Studies as Topic CELLS Biophysics Alkaline phosphatase OSTEOBLAST PHENOTYPE MATRIX Biomedical engineering |
| Popis: | Polyphosphazenes with amino acid ester as side groups are biocompatible polymers that could provide valid scaffolds for cell growth. In the present study we investigate the adhesion and growth of osteoblasts obtained from rat bone marrow on matrices composed of thin fibers of poly[bis(ethyl alanato)phosphazene] (PAlaP), poly(d,l-lactic acid) (PDLLA), or PAlaP/PDLLA blend. Our data show that scaffolds of PAlaP or PAlaP/PDLLA blend enhanced the cell adhesion and growth in comparison with that observed in cultures seeded on polystyrene tissue culture plates. Although collagenase-digestible protein synthesis remained unchanged, all scaffolds induced a decrease in alkaline phosphatase activity, suggesting that osteoblasts are in the proliferation phase. Both PAlaP and PAlaP blended with PDLLA may represent a new and interesting substrate for bone tissue engineering. |
| Databáze: | OpenAIRE |
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