Cytotoxicity assessment of antibiofouling compounds and by-products in marine bivalve cell cultures
Autor: | Stéphanie Auzoux-Bordenave, M Khalanski, Isabelle Domart-Coulon, Dominique Doumenc |
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Rok vydání: | 2000 |
Předmět: |
Oyster
animal structures biology Dose-Response Relationship Drug Molluscacides Cell Survival General Medicine Toxicology biology.organism_classification Bivalvia Ostreidae Acute toxicity Biochemistry In vivo biology.animal Toxicity Crassostrea Animals Viability assay Cytotoxicity Cells Cultured Water Pollutants Chemical |
Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 14(3) |
ISSN: | 0887-2333 |
Popis: | Short-term primary cell cultures were derived from adult marine bivalve tissues: the heart of oyster Crassostrea gigas and the gill of clam Ruditapes decussatus. These cultures were used as experimental in vitro models to assess the acute cytotoxicity of an organic molluscicide, Mexel-432, used in antibiofouling treatments in industrial cooling water systems. A microplate cell viability assay, based on the enzymatic reduction of tetrazolium dye (MTT) in living bivalve cells, was adapted to test the cytotoxicity of this compound: in both in vitro models, toxicity thresholds of Mexel-432 were compared to those determined in vivo with classic acute toxicity tests. The clam gill cell model was also used to assess the cytotoxicity of by-products of chlorination, a major strategy of biofouling control in the marine environment. The applications and limits of these new in vitro models for monitoring aquatic pollutants were discussed, in reference with the standardized Microtox test. |
Databáze: | OpenAIRE |
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