Allergen cleavage by effector cell‐derived proteases regulates allergic inflammation

Autor: Ingrid Rauter, Maria‐Theresa Krauth, Sabine Flicker, Anna Gieras, Kerstin Westritschnig, Susanne Vrtala, Nadja Balic, Susanne Spitzauer, Johannes Huss‐Marp, Knut Brockow, Ulf Darsow, Johannes Ring, Heidrun Behrendt, Hans Semper, Peter Valent, Rudolf Valenta
Rok vydání: 2006
Předmět:
Zdroj: The FASEB Journal. 20:967-969
ISSN: 1530-6860
0892-6638
DOI: 10.1096/fj.05-3999fje
Popis: The key event of allergic inflammation, allergen-induced crosslinking of mast cell-bound IgE antibodies, is accompanied by release of inflammatory mediators, cytokines, and proteases, in particular beta-tryptase. We provide evidence that protease-mediated cleavage of allergens represents a mechanism that regulates allergen-induced mast cell activation. When used in molar ratios as they occur in vivo, purified beta-tryptase cleaved major grass and birch pollen allergens, resulting in defined peptide fragments as mapped by mass spectrometry. Tryptase-cleaved allergens showed reduced IgE reactivity and allergenic activity. The biological relevance is demonstrated by the fact that lysates from activated human mast cells containing tryptase levels as they occur in vivo cleaved allergens. Additionally, protamine, an inhibitor of heparin-dependent effector cell proteases, augmented allergen-induced release of mediators from effector cells. Protease-mediated allergen cleavage may represent an important mechanism for terminating allergen-induced effector cell activation.
Databáze: OpenAIRE
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